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APC Rabbit anti-Mouse CD32 mAb (A26143)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - APC Rabbit anti-Mouse CD32 mAb (A26143)

Flow cytometry: 1X10^6 bEnd.3 cells (negative control, left) and C57BL/6 mouse splenocytes (right) were surface-stained with APC Rabbit anti-Mouse CD32 mAb (A26143, 5 μl/Test, orange line) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - APC Rabbit anti-Mouse CD32 mAb (A26143)

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Mouse CD32 mAb (A26143, 5 μl/Test, right).

ABclonal:Flow CytoMetry - APC Rabbit anti-Mouse CD32 mAb (A26143)

Flow cytometry:1X10^6 C57BL/6 mouse splenocytes were surface-stained with PE Rabbit anti-Mouse CD19 mAb (A25265, 5 μl/Test) and APC Rabbit anti-Mouse CD32 mAb (A26143, 5 μl/Test, right) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, left).

Overview

Product nameAPC Rabbit anti-Mouse CD32 mAb
Catalog No.A26143
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC68229-APC
CD32 (Fcgr2) is a 40 kD transmembrane glycoprotein, member of the immunoglobulin superfamily. The extracellular region of CD32 consists of two Ig C-type domains that binds the Fc region from monomeric IgG with low affinity, but binds immune complexes efficiently. CD32 can mediate phagocytosis of immune complexes and modulate cell activation. CD32 is expressed by Macrophages, neutrophils, mast cells and B cells.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 30-207 of mouse CD32 (NP_034317.1).
SequenceTHDLPKAVVKLEPPWIQVLKEDTVTLTCEGTHNPGNSSTQWFHNGRSIRSQVQASYTFKATVNDSGEYRCQMEQTRLSDPVDLGVISDWLLLQTPQLVFLEGETITLRCHSWRNKLLNRISFFHNEKSVRYHHYSSNFSIPKANHSHSGDYYCKGSLGRTLHQSKPVTITVQGPKSSR
Gene ID
Swiss Prot
SynonymsCD32; Fcgr2; Fcr-2; Fcr-3; Ly-17; LyM-1; Lym-1; fcRII; FcgRII; Fcgr2a; Ly-m20; Fc[g]RII; F630109E10Rik
Calculated MW31kDa/33kDa/36kDa
Observed MWRefer to figures
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Cytoplasm, cytoskeleton, Secreted

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - APC Rabbit anti-Mouse CD32 mAb (A26143)}

    Flow CytoMetry - APC Rabbit anti-Mouse CD32 mAb (A26143)

    Flow cytometry: 1X10^6 bEnd.3 cells (negative control, left) and C57BL/6 mouse splenocytes (right) were surface-stained with APC Rabbit anti-Mouse CD32 mAb (A26143, 5 μl/Test, orange line) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - APC Rabbit anti-Mouse CD32 mAb (A26143)}

    Flow CytoMetry - APC Rabbit anti-Mouse CD32 mAb (A26143)

    Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Mouse CD32 mAb (A26143, 5 μl/Test, right).
    ABclonal:Flow CytoMetry - APC Rabbit anti-Mouse CD32 mAb (A26143)}

    Flow CytoMetry - APC Rabbit anti-Mouse CD32 mAb (A26143)

    Flow cytometry:1X10^6 C57BL/6 mouse splenocytes were surface-stained with PE Rabbit anti-Mouse CD19 mAb (A25265, 5 μl/Test) and APC Rabbit anti-Mouse CD32 mAb (A26143, 5 μl/Test, right) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, left).

    * For research use only. Not for therapeutic or diagnostic purposes.

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