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ATG12 Rabbit mAb (A19610)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATG12 Rabbit mAb (A19610)

Western blot analysis of extracts of various cell lines, using ATG12 antibody (A19610) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product nameATG12 Rabbit mAb
Catalog No.A19610
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0089
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 41-140 of human Apg12 (O94817).
SequenceSPGTEEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG
Gene ID
Swiss Prot
SynonymsAPG12; FBR93; APG12L; HAPG12
Calculated MW15kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesPC-3, Mouse brain, Mouse stomach
Cellular locationCytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane
Customer validation

WB(Rattus norvegicus, Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - ATG12 Rabbit mAb (A19610)}

Western blot - ATG12 Rabbit mAb (A19610)

Western blot analysis of extracts of various cell lines, using ATG12 antibody (A19610) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A19610? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)