제품 > 항체 > 단일클론항체(mAb)

Aggrecan Rabbit mAb (A11691)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of lysates from Mouse lung, using Aggrecan Rabbit mAb (A11691) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Aggrecan Rabbit mAb (A11691)

Immunohistochemistry analysis of paraffin-embedded Rat bone marrow using Aggrecan Rabbit mAb (A11691) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse bone marrow using Aggrecan Rabbit mAb (A11691) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product name	Aggrecan Rabbit mAb
Catalog No.	A11691
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0678

Background

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 761-861 of human Aggrecan (P16112).
Sequence	WPPTGAATEESTEGPSATEVPSASEEPSPSEVPFPSEEPSPSEEPFPSVRPFPSVELFPSEEPFPSKEPSPSEEPSASEEPYTPSPPVPSWTELPSSGEES
Gene ID
Swiss Prot
Synonyms	AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; SSOAOD; Aggrecan
Calculated MW	261kDa
Observed MW	261kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:4000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Mouse lung
Cellular location	Secreted, extracellular matrix, extracellular space.
Customer validation	WB(Mus musculus, Rattus norvegicus, Homo sapiens, Gallus gallus) IHC(Mus musculus, Homo sapiens, Rattus norvegicus) IF(Gallus gallus) IF(Homo sapiens, Rattus norvegicus) IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

연구줄기세포간엽줄기세포

Aggrecan Rabbit mAb images

Western blot - Aggrecan Rabbit mAb (A11691)

Immunohistochemistry - Aggrecan Rabbit mAb (A11691)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

Clin Transl Med

Application:

IF:

11.492

Species:

Mus musculus

PMID:

33463060

Title:

The umbilical cord mesenchymal stem cell‐derived exosomal lncRNA H19 improves osteochondral activity through miR‐29b‐3p/FoxO3 axis

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

Free Radic Biol Med

Application:

IF:

7.376

Species:

Rattus norvegicus

PMID:

34737144

Title:

Glucagon-like peptide-1 receptor activation maintains extracellular matrix integrity by inhibiting the activity of mitogen-activated protein kinases and activator protein-1

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

Arthritis research & therapy

Application:

IF:

5.6

Species:

Homo sapiens

PMID:

36068615

Title:

Circ0007042 alleviates intervertebral disc degeneration by adsorbing miR-369 to upregulate BMP2 and activate the PI3K/AKt pathway - PMC

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

Cell death & disease

Application:

IHC

IF:

9.68

Species:

Mus musculus

PMID:

35739102

Title:

Pentraxin 3 regulated by miR-224-5p modulates macrophage reprogramming and exacerbates osteoarthritis associated synovitis by targeting CD32 - PMC

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

Journal of hazardous materials

Application:

IF:

14.22

Species:

Gallus gallus

PMID:

36423455

Title:

Thiram-induced hyperglycemia causes tibial dyschondroplasia by triggering aberrant ECM remodeling via the gut-pancreas axis in broiler chickens

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

American journal of physiology. Cell physiology

Application:

IHC

IF:

5.28

Species:

Mus musculus

PMID:

36939200

Title:

MiR146a modulates chondrogenesis of bone marrow mesenchymal stem cells by modulating Lsm11 expression

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

Advanced healthcare materials

Application:

IF:

11.09

Species:

Mus musculus

PMID:

37696643

Title:

Retuning Mitochondrial Apoptosis/Mitophagy Balance via SIRT3energized And MicroenvironmentModulated Hydrogel Microspheres to Impede Osteoarthritis

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IHC, IF

IF:

17.52

Species:

Homo sapiens

PMID:

36683245

Title:

Reprogramming of Mitochondrial Respiratory Chain Complex by Targeting SIRT3COX4I2 Axis Attenuates Osteoarthritis Progression

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

Heliyon

Application:

WB, IF

IF:

3.77

Species:

Homo sapiens

PMID:

37771529

Title:

Curcumin reverses erastin-induced chondrocyte ferroptosis by upregulating Nrf2 - PMC

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

Bone research

Application:

IF:

12.7

Species:

Mus musculus

PMID:

38433252

Title:

Targeted knockdown of PGAM5 in synovial macrophages efficiently alleviates osteoarthritis - PMC

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

Journal of translational medicine

Application:

IF:

7.4

Species:

Rattus norvegicus

PMID:

38493143

Title:

Low-intensity pulsed ultrasound delays the progression of osteoarthritis by regulating the YAP-RIPK1-NF-B axis via influencing autophagy

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

Biochemical and biophysical research communications

Application:

IHC

IF:

3.32

Species:

Mus musculus

PMID:

37542771

Title:

Pentraxin 3 deficiency ameliorates the severity of osteoarthritis and alleviates inflammation

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

International journal of biological macromolecules

Application:

WB, IF

IF:

8.02

Species:

Rattus norvegicus

PMID:

37553034

Title:

Fucoidan-functionalized gelatin methacryloyl microspheres ameliorate intervertebral disc degeneration by restoring redox and matrix homeostasis of nucleus

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

Arthritis research & therapy

Application:

IHC

IF:

5.6

Species:

Mus musculus

PMID:

37946278

Title:

Rspo2 exacerbates rheumatoid arthritis by targeting aggressive phenotype of fibroblast-like synoviocytes and disrupting chondrocyte homeostasis via Wnt/-catenin pathway - PMC

References for Aggrecan Rabbit mAb (A11691)

Product:

Aggrecan Rabbit mAb

Journal:

Journal of nanobiotechnology

Application:

IHC

IF:

10.2

Species:

Mus musculus

PMID: