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CD123 Rabbit mAb (A26137)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - CD123 Rabbit mAb (A26137)

Flow cytometry: 1X10^6 RAW264.7 cells (Low Expression, left) and C57BL/6 mouse bone marrow cells (right) were surface-stained with CD123 Rabbit mAb (A26137, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - CD123 Rabbit mAb (A26137)

Flow cytometry: 1X10^6 C57BL/6 mouse bone marrow cells were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or CD123 Rabbit mAb (A26137, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.

Overview

Product nameCD123 Rabbit mAb
Catalog No.A26137
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC65499
Enables interleukin-3 binding activity and interleukin-3 receptor activity. Acts upstream of or within interleukin-3-mediated signaling pathway; monocyte differentiation; and regulation of cell growth. Located in membrane. Is expressed in several structur
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 17-331 of mouse CD123 (NP_032395.1).
SequenceSDLAAVREAPPTAVTTPIQNLHIDPAHYTLSWDPAPGADITTGAFCRKGRDIFVWADPGLARCSFQSLSLCHVTNFTVFLGKDRAVAGSIQFPPDDDGDHEAAAQDLRCWVHEGQLSCQWERGPKATGDVHYRMFWRDVRLGPAHNRECPHYHSLDVNTAGPAPHGGHEGCTLDLDTVLGSTPNSPDLVPQVTITVNGSGRAGPVPCMDNTVDLQRAEVLAPPTLTVECNGSEAHARWVARNRFHHGLLGYTLQVNQSSRSEPQEYNVSIPHFWVPNAGAISFRVKSRSEVYPRKLSSWSEAWGLVCPPEVMPVK
Gene ID
Swiss Prot
SynonymsCD123; SUT-1; CDw123
Calculated MW43kDa
Observed MWRefer to figures
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Endomembrane system

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - CD123 Rabbit mAb (A26137)}

    Flow CytoMetry - CD123 Rabbit mAb (A26137)

    Flow cytometry: 1X10^6 RAW264.7 cells (Low Expression, left) and C57BL/6 mouse bone marrow cells (right) were surface-stained with CD123 Rabbit mAb (A26137, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - CD123 Rabbit mAb (A26137)}

    Flow CytoMetry - CD123 Rabbit mAb (A26137)

    Flow cytometry: 1X10^6 C57BL/6 mouse bone marrow cells were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or CD123 Rabbit mAb (A26137, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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