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CD162/PSGL-1 Rabbit mAb (A25952)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using CD162/PSGL-1 Rabbit mAb (A25952) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using CD162/PSGL-1 Rabbit mAb (A25952) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using CD162/PSGL-1 Rabbit mAb (A25952) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - CD162/PSGL-1 Rabbit mAb (A25952)

Confocal imaging of paraffin-embedded Mouse spleen tissue using CD162/PSGL-1 Rabbit mAb (A25952, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Confocal imaging of paraffin-embedded Mouse thymus tissue using CD162/PSGL-1 Rabbit mAb (A25952, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Flow CytoMetry - CD162/PSGL-1 Rabbit mAb (A25952)

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and C57BL/6 mouse splenocytes (right) were surface-stained with CD162/PSGL-1 Rabbit mAb (A25952, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by FITC conjugated goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Overview

Product name	CD162/PSGL-1 Rabbit mAb
Catalog No.	A25952
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC66570

Background

Involved in leukocyte adhesive activation. Acts upstream of or within leukocyte tethering or rolling. Predicted to be located in plasma membrane raft and uropod. Predicted to be active in plasma membrane. Is expressed in brain and thymus primordium. Human ortholog(s) of this gene implicated in carotid artery disease. Orthologous to human SELPLG (selectin P ligand).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 18-320 of mouse CD162/PSGL-1 (NP_033177.3).
Sequence	LQLQDPWGHETKEAPGPVHLRERRQVVGDDDFEDPDYTYNTDPPELLKNVTNTVAAHPELPTTVVMLERDSTSAGTSERATEKIATTDPTAPGTGGTAVGMLSTDSATQWSLTSVETVQPASTEVETSQPAPMEAETSQPAPMEAETSQPAPMEAETSQPAPMEADTSQPAPMEADTSKPAPTEAETSKPAPTEAETSQPAPNEAETSKPAPTEAETSKPAPTEAETTQLPRIQAVKTLFTTSAATEVPSTEPTTMETASTESNESTIFLGPSVTHLPDSGLKKGLIVTPGNSPAPTLPGSSD
Gene ID
Swiss Prot
Synonyms	CD162; Psgl1; Selp1; Selpl; Psgl-1
Calculated MW	44kDa
Observed MW

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IHC-P 1:200 - 1:2000 IF/ICC 1:200 - 1:2000 FC 1:100 - 1:500 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Immunohistochemistry Immunofluorescence Flow Cytometry
Positive samples
Cellular location	cell membrane.