제품 > 항체 > 단일클론항체(mAb)

PE Rabbit anti-Mouse CD162/PSGL-1 mAb (A25949)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and C57BL/6 mouse splenocytes cells (right) were surface-stained with PE Rabbit anti-Mouse CD162/PSGL-1 mAb (A25949, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Mouse CD162/PSGL-1 mAb (A25949, 5 μl/Test, right).

Review

Overview

Product name	PE Rabbit anti-Mouse CD162/PSGL-1 mAb
Catalog No.	A25949
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC66571-PE

Background

Involved in leukocyte adhesive activation. Acts upstream of or within leukocyte tethering or rolling. Predicted to be located in plasma membrane raft and uropod. Predicted to be active in plasma membrane. Is expressed in brain and thymus primordium. Human ortholog(s) of this gene implicated in carotid artery disease. Orthologous to human SELPLG (selectin P ligand).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 18-320 of mouse CD162/PSGL-1 (NP_033177.3).
Sequence	LQLQDPWGHETKEAPGPVHLRERRQVVGDDDFEDPDYTYNTDPPELLKNVTNTVAAHPELPTTVVMLERDSTSAGTSERATEKIATTDPTAPGTGGTAVGMLSTDSATQWSLTSVETVQPASTEVETSQPAPMEAETSQPAPMEAETSQPAPMEAETSQPAPMEADTSQPAPMEADTSKPAPTEAETSKPAPTEAETSQPAPNEAETSKPAPTEAETSKPAPTEAETTQLPRIQAVKTLFTTSAATEVPSTEPTTMETASTESNESTIFLGPSVTHLPDSGLKKGLIVTPGNSPAPTLPGSSD
Gene ID
Swiss Prot
Synonyms	CD162; Psgl1; Selp1; Selpl; Psgl-1
Calculated MW	44kDa
Observed MW

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	cell membrane
Customer validation	(Mouse bone marrow)