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CD79a Rabbit pAb (A0331)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CD79a Rabbit pAb (A0331)

Western blot analysis of lysates from Raji cells, using CD79a Rabbit pAb (A0331) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - CD79a Rabbit pAb (A0331)

Western blot analysis of various lysates using CD79a Rabbit pAb (A0331) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - CD79a Rabbit pAb (A0331)

Immunohistochemistry analysis of paraffin-embedded rat spleen using CD79a Rabbit pAb (A0331) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CD79a Rabbit pAb (A0331)

Immunohistochemistry analysis of paraffin-embedded human spleen using CD79a Rabbit pAb (A0331) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CD79a Rabbit pAb (A0331)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD79a Rabbit pAb (A0331) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - CD79a Rabbit pAb (A0331)

Immunofluorescence analysis of paraffin-embedded rat spleen using CD79a Rabbit pAb (A0331) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD79a Rabbit pAb (A0331)

Immunofluorescence analysis of paraffin-embedded mouse spleen using CD79a Rabbit pAb (A0331) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCD79a Rabbit pAb
Catalog No.A0331
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 165-225 of human CD79a (NP_001774.1).
SequenceFRKRWQNEKLGLDAGDEYEDENLYEGLNLDDCSMYEDISRGLQGTYQDVGSLNIGDVQLEK
Gene ID
Swiss Prot
SynonymsIGA; MB1; MB-1; IGAlpha; CD79a
Calculated MW25kDa
Observed MW40-50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRaji, Mouse thymus, Rat liver
Cellular locationCell membrane, Single-pass type I membrane protein
Customer validation

IF(Sus scrofa)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - CD79a Rabbit pAb (A0331)}

Western blot - CD79a Rabbit pAb (A0331)

Western blot analysis of lysates from Raji cells, using CD79a Rabbit pAb (A0331) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - CD79a Rabbit pAb (A0331)}

Western blot - CD79a Rabbit pAb (A0331)

Western blot analysis of various lysates using CD79a Rabbit pAb (A0331) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - CD79a Rabbit pAb (A0331)}

Immunohistochemistry - CD79a Rabbit pAb (A0331)

Immunohistochemistry analysis of paraffin-embedded rat spleen using CD79a Rabbit pAb (A0331) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CD79a Rabbit pAb (A0331)}

Immunohistochemistry - CD79a Rabbit pAb (A0331)

Immunohistochemistry analysis of paraffin-embedded human spleen using CD79a Rabbit pAb (A0331) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CD79a Rabbit pAb (A0331)}

Immunohistochemistry - CD79a Rabbit pAb (A0331)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD79a Rabbit pAb (A0331) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - CD79a Rabbit pAb (A0331)}

Immunofluorescence - CD79a Rabbit pAb (A0331)

Immunofluorescence analysis of paraffin-embedded rat spleen using CD79a Rabbit pAb (A0331) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD79a Rabbit pAb (A0331)}

Immunofluorescence - CD79a Rabbit pAb (A0331)

Immunofluorescence analysis of paraffin-embedded mouse spleen using CD79a Rabbit pAb (A0331) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0331? Please let us know so that we can cite the reference in this datasheet.

항체 (7)

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Secondary Antibodies (26)