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CD81 Rabbit PolymAb® (A22986PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CD81 Rabbit PolymAb® (A22986PM)

Western blot analysis of various lysates, using CD81 Rabbit PolymAb® (A22986PM) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - CD81 Rabbit PolymAb® (A22986PM)

Immunohistochemistry analysis of paraffin-embedded Human liver using CD81 Rabbit PolymAb® (A22986PM) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD81 Rabbit PolymAb® (A22986PM)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using CD81 Rabbit PolymAb® (A22986PM) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD81 Rabbit PolymAb® (A22986PM)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using CD81 Rabbit PolymAb® (A22986PM) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameCD81 Rabbit PolymAb®
Catalog No.A22986PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 114-199 of human CD81(NP_004347.1).
SequenceVNKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFS
Gene ID
Swiss Prot
SynonymsS5.7; CVID6; TAPA1; TSPAN28
Calculated MW26kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRaji, Mouse brain, Rat brain
Cellular locationMembrane.
ABclonal:Western blot - CD81 Rabbit PolymAb® (A22986PM)}

Western blot - CD81 Rabbit PolymAb® (A22986PM)

Western blot analysis of various lysates, using CD81 Rabbit PolymAb® (A22986PM) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - CD81 Rabbit PolymAb® (A22986PM)}

Immunohistochemistry - CD81 Rabbit PolymAb® (A22986PM)

Immunohistochemistry analysis of paraffin-embedded Human liver using CD81 Rabbit PolymAb® (A22986PM) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD81 Rabbit PolymAb® (A22986PM)}

Immunohistochemistry - CD81 Rabbit PolymAb® (A22986PM)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using CD81 Rabbit PolymAb® (A22986PM) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD81 Rabbit PolymAb® (A22986PM)}

Immunohistochemistry - CD81 Rabbit PolymAb® (A22986PM)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using CD81 Rabbit PolymAb® (A22986PM) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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