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CD81 Rabbit mAb (A22528)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - CD81 Rabbit mAb (A22528)

Western blot analysis of various lysates, using CD81 Rabbit mAb (A22528) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - CD81 Rabbit mAb (A22528)

Western blot analysis of lysates from Rat brain, using CD81 Rabbit mAb (A22528) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - CD81 Rabbit mAb (A22528)

Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using CD81 Rabbit mAb (A22528) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD81 Rabbit mAb (A22528)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD81 Rabbit mAb (A22528) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD81 Rabbit mAb (A22528)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using CD81 Rabbit mAb (A22528) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD81 Rabbit mAb (A22528)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using CD81 Rabbit mAb (A22528) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameCD81 Rabbit mAb
Catalog No.A22528
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC57928
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 114-199 of human CD81 (NP_004347.1).
SequenceVNKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFS
Gene ID
Swiss Prot
SynonymsS5.7; CVID6; TAPA1; TSPAN28; CD81
Calculated MW26kDa
Observed MW20kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRaji, Rat brain
Cellular locationMembrane, Multi-pass membrane protein.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - CD81 Rabbit mAb (A22528)}

Western blot - CD81 Rabbit mAb (A22528)

Western blot analysis of various lysates, using CD81 Rabbit mAb (A22528) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - CD81 Rabbit mAb (A22528)}

Western blot - CD81 Rabbit mAb (A22528)

Western blot analysis of lysates from Rat brain, using CD81 Rabbit mAb (A22528) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - CD81 Rabbit mAb (A22528)}

Immunohistochemistry - CD81 Rabbit mAb (A22528)

Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using CD81 Rabbit mAb (A22528) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD81 Rabbit mAb (A22528)}

Immunohistochemistry - CD81 Rabbit mAb (A22528)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD81 Rabbit mAb (A22528) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD81 Rabbit mAb (A22528)}

Immunohistochemistry - CD81 Rabbit mAb (A22528)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using CD81 Rabbit mAb (A22528) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD81 Rabbit mAb (A22528)}

Immunohistochemistry - CD81 Rabbit mAb (A22528)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using CD81 Rabbit mAb (A22528) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A22528? Please let us know so that we can cite the reference in this datasheet.

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Secondary Antibodies (26)