제품 > 항체 > 단일클론항체(mAb)

CREB Rabbit mAb (A10826)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of various lysates using CREB Rabbit mAb (A10826) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product name	CREB Rabbit mAb
Catalog No.	A10826
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0113

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human CREB1 (P16220).
Sequence	ATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENR
Gene ID
Swiss Prot
Synonyms	CREB; CREB-1
Calculated MW	35kDa
Observed MW	43kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse lung, Mouse brain, Rat brain
Cellular location	Nucleus.
Customer validation	IF(Mus musculus) WB(Rattus norvegicus, Homo sapiens, Mus musculus, Mus musculus, Other) IHC(Homo sapiens) IF(Homo sapiens, Mus musculus) IP(Homo sapiens, Mus musculus)

Research Area

CREB Rabbit mAb images

Western blot - CREB Rabbit mAb (A10826)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for CREB Rabbit mAb (A10826)

Product:

CREB Rabbit mAb

Journal:

Inflammation

Application:

IF:

4.5

Species:

Mus musculus

PMID:

33067662

Title:

CGRP-Mediated Prolactin Upregulation: a Possible Pathomechanism in IgG4-Related Disease

References for CREB Rabbit mAb (A10826)

Product:

CREB Rabbit mAb

Journal:

Oxid Med Cell Longev

Application:

IF:

Species:

Rattus norvegicus

PMID:

34646421

Title:

Jie-Yu-He-Huan Capsule Ameliorates Anxiety-Like Behaviours in Rats Exposed to Chronic Restraint Stress via the cAMP/PKA/CREB/BDNF Signalling Pathway

References for CREB Rabbit mAb (A10826)

Product:

CREB Rabbit mAb

Journal:

Nature communications

Application:

IF:

16.6

Species:

Homo sapiens

PMID:

36443308

Title:

Secreted EMC10 is upregulated in human obesity and its neutralizing antibody prevents diet-induced obesity in mice - PMC

References for CREB Rabbit mAb (A10826)

Product:

CREB Rabbit mAb

Journal:

Oxid Med Cell Longev

Application:

WB, IHC, IF

IF:

Species:

Homo sapiens

PMID:

34394823

Title:

Methylation of CALCA and CALCB in Pancreatic Ductal Adenocarcinoma

References for CREB Rabbit mAb (A10826)

Product:

CREB Rabbit mAb

Journal:

Andrology

Application:

IF:

4.6

Species:

Rattus norvegicus

PMID:

36762774

Title:

Low‐intensity pulsed ultrasound ameliorates erectile dysfunction induced by bilateral cavernous nerve injury through enhancing schwann cell‐mediated cavernous …

References for CREB Rabbit mAb (A10826)

Product:

CREB Rabbit mAb

Journal:

Frontiers in microbiology

Application:

IF:

6.1

Species:

Mus musculus

PMID:

37089558

Title:

Gut microbiota from sigma-1 receptor knockout mice induces depression-like behaviors and modulates the cAMP/CREB/BDNF signaling pathway - PMC

References for CREB Rabbit mAb (A10826)

Product:

CREB Rabbit mAb

Journal:

Biology

Application:

IF:

4.2

Species:

Rattus norvegicus

PMID:

37372046

Title:

Brain-Derived Estrogen Regulates Neurogenesis, Learning and Memory with Aging in Female Rats - PMC

References for CREB Rabbit mAb (A10826)

Product:

CREB Rabbit mAb

Journal:

Cell Death Dis

Application:

WB, IP

IF:

8.1

Species:

Homo sapiens, Mus musculus

PMID:

33040080

Title:

USP7 mediates pathological hepatic de novo lipogenesis through promoting stabilization and transcription of ZNF638

References for CREB Rabbit mAb (A10826)

Product:

CREB Rabbit mAb

Journal:

Cell Proliferation

Application:

WB, IF

IF:

8.5

Species:

Homo sapiens, Mus musculus

PMID:

31418947

Title:

Inhibition of CREB-mediated ZO-1 and Activation of NF-B-induced IL-6 by Colonic Epithelial MCT4 Destroys Intestinal Barrier Function.

References for CREB Rabbit mAb (A10826)

Product:

CREB Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

37678029

Title:

Inflammatory regulation by restraining M2 microglial polarization: Neurodestructive effects of Kallikrein-related peptidase 8 activation in intracerebral hemorrhage

References for CREB Rabbit mAb (A10826)

Product:

CREB Rabbit mAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

6.2

Species:

Mus musculus

PMID:

38157803

Title:

Effect of chronic noise exposure on glucose and lipid metabolism in mice via modulating gut microbiota and regulating CREB/CRTC2 and SREBP1/SCD

References for CREB Rabbit mAb (A10826)

Product:

CREB Rabbit mAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

6.9

Species:

Homo sapiens

PMID:

38113631

Title:

Berberine exerts antidepressant effects in vivo and in vitro through the PI3K/AKT/CREB/BDNF signaling pathway

References for CREB Rabbit mAb (A10826)

Product:

CREB Rabbit mAb

Journal:

Neural plasticity

Application:

IF:

3.1

Species:

Rattus norvegicus

PMID:

36910013

Title:

Electroacupuncture Zusanli (ST36) Relieves Somatic Pain in Colitis Rats by Inhibiting Dorsal Root Ganglion Sympathetic-Sensory Coupling and Neurogenic Inflammation - PMC

References for CREB Rabbit mAb (A10826)

Product:

CREB Rabbit mAb

Journal:

Journal of ethnopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

38296172