Reviews (2)Publications (7) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CREB1-S133 Rabbit pAb (AP0333) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-CREB1-S133 Rabbit pAb (AP0333) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Phospho-CREB1-S133 Rabbit pAb (AP0333) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using Phospho-CREB1-S133 Rabbit pAb (AP0333) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
| Product name | Phospho-CREB1-S133 Rabbit pAb |
|---|---|
| Catalog No. | AP0333 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around S133 of human CREB1 (NP_604391.1). |
|---|---|
| Sequence | RPSYR |
| Gene ID | |
| Swiss Prot | |
| Synonyms | CREB; CREB-1; Phospho-CREB1-S133 |
| Calculated MW | 35kDa |
| Observed MW | 40kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | NIH/3T3 treated by UV or Anisomycin |
| Cellular location | Nucleus. |
| Customer validation | (Mouse kidney) IF(Mus musculus) WB(Homo sapiens, Mus musculus, Mus musculus) IHC(Homo sapiens) IF(Homo sapiens) |
To download a Certificate of Compliance, please enter your Lot number below:
Lot number
* For research use only. Not for therapeutic or diagnostic purposes.
Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr
Publishing research using AP0333? Please let us know so that we can cite the reference in this datasheet.
_WB_01.jpg?t=1718354983 "ABclonal:Western blot - Phospho-CREB1-S133 Rabbit pAb (AP0333)")
_IHC_02.jpg?t=1718354983 "ABclonal:Immunohistochemistry - Phospho-CREB1-S133 Rabbit pAb (AP0333)")
_IHC_01.jpg?t=1718354983 "ABclonal:Immunohistochemistry - Phospho-CREB1-S133 Rabbit pAb (AP0333)")
_IHC_03.jpg?t=1718354983 "ABclonal:Immunohistochemistry - Phospho-CREB1-S133 Rabbit pAb (AP0333)")
_WB_01.jpg?t=1716196302)
_WB_01.jpg?t=1718355793)
