Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates, using DNA Ligase III/LIG3 antibody (A22136) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
Immunohistochemistry analysis of DNA Ligase III/LIG3 in paraffin-embedded human cervix cancer tissue using DNA Ligase III/LIG3 Rabbit mAb (A22136) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of DNA Ligase III/LIG3 in paraffin-embedded human colon tissue using DNA Ligase III/LIG3 Rabbit mAb (A22136) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of DNA Ligase III/LIG3 in paraffin-embedded mouse colon tissue using DNA Ligase III/LIG3 Rabbit mAb (A22136) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of DNA Ligase III/LIG3 in paraffin-embedded mouse testis tissue using DNA Ligase III/LIG3 Rabbit mAb (A22136) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of DNA Ligase III/LIG3 in paraffin-embedded rat colon tissue using DNA Ligase III/LIG3 Rabbit mAb (A22136) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of DNA Ligase III/LIG3 in paraffin-embedded rat kidney tissue using DNA Ligase III/LIG3 Rabbit mAb (A22136) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
| Product name | DNA Ligase III/LIG3 Rabbit mAb |
|---|---|
| Catalog No. | A22136 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC55533 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human DNA Ligase III/LIG3 (NP_039269.2). |
|---|---|
| Sequence | IASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACKSVEYAMKKCP |
| Gene ID | |
| Swiss Prot | |
| Synonyms | LIG2; MTDPS20; LIG3alpha |
| Calculated MW | 113kDa |
| Observed MW | 96kDa(DNA Ligase III β); 103kDa(DNA Ligase III α) |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | U-87MG, Jurkat, 293F, Mouse testis, Rat testis |
| Cellular location | cytoplasm, mitochondrion, nucleoplasm, nucleus |
| Customer validation | WB(Rattus norvegicus) IHC(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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