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DNA Ligase III/LIG3 Rabbit pAb (A13507)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DNA Ligase III/LIG3 Rabbit pAb (A13507)

Western blot analysis of various lysates using (A13507) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - DNA Ligase III/LIG3 Rabbit pAb (A13507)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using DNA Ligase III/LIG3 Rabbit pAb (A13507) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - DNA Ligase III/LIG3 Rabbit pAb (A13507)

Immunohistochemistry analysis of paraffin-embedded Rat brain using DNA Ligase III/LIG3 Rabbit pAb (A13507) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - DNA Ligase III/LIG3 Rabbit pAb (A13507)

Immunohistochemistry analysis of paraffin-embedded Rat liver using DNA Ligase III/LIG3 Rabbit pAb (A13507) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - DNA Ligase III/LIG3 Rabbit pAb (A13507)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using DNA Ligase III/LIG3 Rabbit pAb (A13507) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - DNA Ligase III/LIG3 Rabbit pAb (A13507)

Immunofluorescence analysis of NIH/3T3 cells using DNA Ligase III/LIG3 Rabbit pAb (A13507) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DNA Ligase III/LIG3 Rabbit pAb (A13507)

Immunofluorescence analysis of PC-12 cells using DNA Ligase III/LIG3 Rabbit pAb (A13507) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameDNA Ligase III/LIG3 Rabbit pAb
Catalog No.A13507
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 254-460 of human DNA Ligase III/LIG3 (NP_039269.2).
SequenceCDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQE
Gene ID
Swiss Prot
SynonymsLIG2; MTDPS20; LIG3alpha; DNA Ligase III/LIG3
Calculated MW113kDa
Observed MW105kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Mouse testis, Rat testis
Cellular locationMitochondrion, Mitochondrion, Nucleus.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - DNA Ligase III/LIG3 Rabbit pAb (A13507)}

    Western blot - DNA Ligase III/LIG3 Rabbit pAb (A13507)

    Western blot analysis of various lysates using (A13507) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - DNA Ligase III/LIG3 Rabbit pAb (A13507)}

    Immunohistochemistry - DNA Ligase III/LIG3 Rabbit pAb (A13507)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using DNA Ligase III/LIG3 Rabbit pAb (A13507) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - DNA Ligase III/LIG3 Rabbit pAb (A13507)}

    Immunohistochemistry - DNA Ligase III/LIG3 Rabbit pAb (A13507)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using DNA Ligase III/LIG3 Rabbit pAb (A13507) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - DNA Ligase III/LIG3 Rabbit pAb (A13507)}

    Immunohistochemistry - DNA Ligase III/LIG3 Rabbit pAb (A13507)

    Immunohistochemistry analysis of paraffin-embedded Rat liver using DNA Ligase III/LIG3 Rabbit pAb (A13507) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - DNA Ligase III/LIG3 Rabbit pAb (A13507)}

    Immunohistochemistry - DNA Ligase III/LIG3 Rabbit pAb (A13507)

    Immunohistochemistry analysis of paraffin-embedded Rat ovary using DNA Ligase III/LIG3 Rabbit pAb (A13507) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - DNA Ligase III/LIG3 Rabbit pAb (A13507)}

    Immunofluorescence - DNA Ligase III/LIG3 Rabbit pAb (A13507)

    Immunofluorescence analysis of NIH/3T3 cells using DNA Ligase III/LIG3 Rabbit pAb (A13507) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - DNA Ligase III/LIG3 Rabbit pAb (A13507)}

    Immunofluorescence - DNA Ligase III/LIG3 Rabbit pAb (A13507)

    Immunofluorescence analysis of PC-12 cells using DNA Ligase III/LIG3 Rabbit pAb (A13507) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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