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DNA-PKcs Rabbit pAb (A7716)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - DNA-PKcs Rabbit pAb (A7716)

Western blot analysis of various lysates using DNA-PKcs Rabbit pAb (A7716) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - DNA-PKcs Rabbit pAb (A7716)

Immunohistochemistry analysis of paraffin-embedded Human well-differentiated squamous skin carcinoma using DNA-PKcs Rabbit pAb (A7716) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - DNA-PKcs Rabbit pAb (A7716)

Immunohistochemistry analysis of paraffin-embedded Human kidney cancer using DNA-PKcs Rabbit pAb (A7716) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - DNA-PKcs Rabbit pAb (A7716)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using DNA-PKcs Rabbit pAb (A7716) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - DNA-PKcs Rabbit pAb (A7716)

Immunofluorescence analysis of U-2OS cells using DNA-PKcs Rabbit pAb (A7716) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameDNA-PKcs Rabbit pAb
Catalog No.A7716
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 3987-4086 of human DNA-PKcs (NP_008835.5).
SequenceALRAFRSDPGLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKD
Gene ID
Swiss Prot
SynonymsHYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNAPKc; DNA-PKC; DNA-PKcs
Calculated MW469kDa
Observed MW469kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, HeLa, A-549
Cellular locationNucleus, nucleolus
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - DNA-PKcs Rabbit pAb (A7716)}

Western blot - DNA-PKcs Rabbit pAb (A7716)

Western blot analysis of various lysates using DNA-PKcs Rabbit pAb (A7716) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - DNA-PKcs Rabbit pAb (A7716)}

Immunohistochemistry - DNA-PKcs Rabbit pAb (A7716)

Immunohistochemistry analysis of paraffin-embedded Human well-differentiated squamous skin carcinoma using DNA-PKcs Rabbit pAb (A7716) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - DNA-PKcs Rabbit pAb (A7716)}

Immunohistochemistry - DNA-PKcs Rabbit pAb (A7716)

Immunohistochemistry analysis of paraffin-embedded Human kidney cancer using DNA-PKcs Rabbit pAb (A7716) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - DNA-PKcs Rabbit pAb (A7716)}

Immunohistochemistry - DNA-PKcs Rabbit pAb (A7716)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using DNA-PKcs Rabbit pAb (A7716) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - DNA-PKcs Rabbit pAb (A7716)}

Immunofluorescence - DNA-PKcs Rabbit pAb (A7716)

Immunofluorescence analysis of U-2OS cells using DNA-PKcs Rabbit pAb (A7716) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A7716? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

Secondary Antibodies (26)