제품 > 항체 > 인산화 특이적 항체

Phospho-DNA-PKcs-S2056 Rabbit pAb (AP0621)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from HeLa cells, using Phospho-DNA-PKcs-S2056 Rabbit pAb (AP0621).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

ABclonal:Immunohistochemistry - Phospho-DNA-PKcs-S2056 Rabbit pAb (AP0621)

Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using Phospho-DNA-PKcs-S2056 Rabbit pAb (AP0621) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-DNA-PKcs-S2056 Rabbit pAb (AP0621)

Immunofluorescence analysis of U2OS cells using Phospho-DNA-PKcs-S2056 Rabbit pAb (AP0621) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Phospho-DNA-PKcs-S2056 Rabbit pAb
Catalog No.	AP0621
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S2056 of human DNA-PKcs (NP_008835.5).
Sequence	YSSQDP
Gene ID
Swiss Prot
Synonyms	HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNAPKc; DNA-PKC; DNA-PKcs; Phospho-DNA-PKcs-S2056
Calculated MW	469kDa
Observed MW	469kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:100 - 1:500 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa
Cellular location	Nucleus, nucleolus.
Customer validation	WB(Homo sapiens, Macaca mulatta)