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EBP1/PA2G4 Rabbit pAb (A5376)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - EBP1/PA2G4  Rabbit pAb (A5376)

Western blot analysis of various lysates using EBP1/PA2G4 Rabbit pAb (A5376) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - EBP1/PA2G4  Rabbit pAb (A5376)

Immunohistochemistry analysis of paraffin-embedded Rat lung using EBP1/PA2G4 Rabbit pAb (A5376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - EBP1/PA2G4  Rabbit pAb (A5376)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using EBP1/PA2G4 Rabbit pAb (A5376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - EBP1/PA2G4  Rabbit pAb (A5376)

Immunohistochemistry analysis of paraffin-embedded Human esophagus using EBP1/PA2G4 Rabbit pAb (A5376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameEBP1/PA2G4 Rabbit pAb
Catalog No.A5376
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-394 of human EBP1/PA2G4 (NP_006182.2).
SequenceMSGEDEQQEQTIAEDLVVTKYKMGGDIANRVLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKEMKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKIDLGVHVDGFIANVAHTFVVDVAQGTQVTGRKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWNKVAHSFNCTPIEGMLSHQLKQHVIDGEKTIIQNPTDQQKKDHEKAEFEVHEVYAVDVLVSSGEGKAKDAGQRTTIYKRDPSKQYGLKMKTSRAFFSEVERRFDAMPFTLRAFEDEKKARMGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMPNGPMRITSGPFEPDLYKSEMEVQDAELKALLQSSASRKTQKKKKKKASKTAENATSGETLEENEAGD
Gene ID
Swiss Prot
SynonymsEBP1; HG4-1; ITAF45; p38-2G4; EBP1/PA2G4
Calculated MW44kDa
Observed MW44kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse liver, BxPC-3, HeLa, PC-12, NIH/3T3, K-562, 22Rv1, HT-29
Cellular locationCytoplasm, Nucleus, nucleolus

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ABclonal:Western blot - EBP1/PA2G4  Rabbit pAb (A5376)}

Western blot - EBP1/PA2G4 Rabbit pAb (A5376)

Western blot analysis of various lysates using EBP1/PA2G4 Rabbit pAb (A5376) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - EBP1/PA2G4  Rabbit pAb (A5376)}

Immunohistochemistry - EBP1/PA2G4 Rabbit pAb (A5376)

Immunohistochemistry analysis of paraffin-embedded Rat lung using EBP1/PA2G4 Rabbit pAb (A5376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - EBP1/PA2G4  Rabbit pAb (A5376)}

Immunohistochemistry - EBP1/PA2G4 Rabbit pAb (A5376)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using EBP1/PA2G4 Rabbit pAb (A5376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - EBP1/PA2G4  Rabbit pAb (A5376)}

Immunohistochemistry - EBP1/PA2G4 Rabbit pAb (A5376)

Immunohistochemistry analysis of paraffin-embedded Human esophagus using EBP1/PA2G4 Rabbit pAb (A5376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)