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[KO Validated] EBP1/PA2G4 Rabbit pAb (A14037)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] EBP1/PA2G4  Rabbit pAb (A14037)

Western blot analysis of extracts of various cell lines, using EBP1/PA2G4 antibody (A14037) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Western blot - [KO Validated] EBP1/PA2G4  Rabbit pAb (A14037)

Western blot analysis of extracts from wild type(WT) and EBP1/PA2G4 knockout (KO) 293T(KO) cells, using EBP1/PA2G4 antibody (A14037) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name[KO Validated] EBP1/PA2G4 Rabbit pAb
Catalog No.A14037
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-394 of human EBP1/PA2G4 (NP_006182.2).
SequenceMSGEDEQQEQTIAEDLVVTKYKMGGDIANRVLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKEMKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKIDLGVHVDGFIANVAHTFVVDVAQGTQVTGRKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWNKVAHSFNCTPIEGMLSHQLKQHVIDGEKTIIQNPTDQQKKDHEKAEFEVHEVYAVDVLVSSGEGKAKDAGQRTTIYKRDPSKQYGLKMKTSRAFFSEVERRFDAMPFTLRAFEDEKKARMGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMPNGPMRITSGPFEPDLYKSEMEVQDAELKALLQSSASRKTQKKKKKKASKTAENATSGETLEENEAGD
Gene ID
Swiss Prot
SynonymsEBP1; HG4-1; ITAF45; p38-2G4
Calculated MW44kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T
Cellular locationCytoplasm, Nucleus, nucleolus

Documents

Certificate of Compliance

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ABclonal:Western blot - [KO Validated] EBP1/PA2G4  Rabbit pAb (A14037)}

Western blot - [KO Validated] EBP1/PA2G4 Rabbit pAb (A14037)

Western blot analysis of extracts of various cell lines, using EBP1/PA2G4 antibody (A14037) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] EBP1/PA2G4  Rabbit pAb (A14037)}

Western blot - [KO Validated] EBP1/PA2G4 Rabbit pAb (A14037)

Western blot analysis of extracts from wild type(WT) and EBP1/PA2G4 knockout (KO) 293T(KO) cells, using EBP1/PA2G4 antibody (A14037) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

재조합 단백질 (1)

Secondary Antibodies (26)