제품 > 항체 > 단일클론항체(mAb)

E-Cadherin Rabbit mAb (A22850)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from Mouse lung, using E-Cadherin Rabbit mAb (A22850) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - E-Cadherin Rabbit mAb (A22850)

Immunohistochemistry analysis of paraffin-embedded Human liver using E-Cadherin Rabbit mAb (A22850) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using E-Cadherin Rabbit mAb (A22850) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using E-Cadherin Rabbit mAb (A22850) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using E-Cadherin Rabbit mAb (A22850) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using E-Cadherin Rabbit mAb (A22850) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human stomach tissue using E-Cadherin Rabbit mAb (A22850) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - E-Cadherin Rabbit mAb (A22850)

Confocal imaging of A-431 cells using E-Cadherin Rabbit mAb (A22850, at dilution of 1:200) (Green). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Red). DAPI was used for nuclear staining (blue). Objective: 100x.

Confocal imaging of MCF7 cells using E-Cadherin Rabbit mAb (A22850, at dilution of 1:200) (Green). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Red). DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product name	E-Cadherin Rabbit mAb
Catalog No.	A22850
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC57093

Background

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.

Immunogen information

Immunogen	Recombinant funsion protein containing a sequence corresponding to amino acids 155-707 of human E-Cadherin (NP_004351.1).
Sequence	DWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQI
Gene ID
Swiss Prot
Synonyms	UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324; E-Cadherin
Calculated MW	97kDa
Observed MW	135kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:500 - 1:2000 IF/ICC 1:200 - 1:800 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Mouse lung
Cellular location	Cell junction, Cell membrane, Endosome, Golgi apparatus, Single-pass type I membrane protein, trans-Golgi network.
Customer validation	WB(Homo sapiens, Bos taurus, Mus musculus) IF(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

E-Cadherin Rabbit mAb images

Western blot - E-Cadherin Rabbit mAb (A22850)

Immunohistochemistry - E-Cadherin Rabbit mAb (A22850)

Immunofluorescence - E-Cadherin Rabbit mAb (A22850)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for E-Cadherin Rabbit mAb (A22850)

Product:

E-Cadherin Rabbit mAb

Journal:

Hematology (Amsterdam, Netherlands)

Application:

IF:

Species:

Homo sapiens

PMID:

37014739

Title:

miR-100-5p is upregulated in multiple myeloma and involves in the pathogenesis of multiple myeloma through targeting MTMR3

References for E-Cadherin Rabbit mAb (A22850)

Product:

E-Cadherin Rabbit mAb

Journal:

Biochemical pharmacology

Application:

IF:

5.3

Species:

Homo sapiens

PMID:

38552852

Title:

TDO2 promotes bladder cancer progression via AhR-mediated SPARC/FILIP1L signaling

References for E-Cadherin Rabbit mAb (A22850)

Product:

E-Cadherin Rabbit mAb

Journal:

Advanced healthcare materials

Application:

IF:

Species:

Mus musculus

PMID:

37669499

Title:

A Zwitterionic Hydrogel with AntiOxidative and AntiInflammatory Properties for the Prevention of Peritoneal Adhesion by Inhibiting MesothelialMesenchymal

References for E-Cadherin Rabbit mAb (A22850)

Product:

E-Cadherin Rabbit mAb

Journal:

Nature communications

Application:

IF:

16.6

Species:

Homo sapiens

PMID:

38862489

Title:

MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration - PMC

References for E-Cadherin Rabbit mAb (A22850)

Product:

E-Cadherin Rabbit mAb

Journal:

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas

Application:

IF:

1.9

Species:

Bos taurus

PMID:

39166606

Title:

GSK3B inhibition reduced cervical cancer cell proliferation and migration by modulating the PI3K/Akt signaling pathway and epithelial-to-mesenchymal transition - PMC

References for E-Cadherin Rabbit mAb (A22850)

Product:

E-Cadherin Rabbit mAb

Journal:

Cell death & disease

Application:

IF:

8.1

Species:

Mus musculus

PMID:

39487118