제품 > 항체 > 다중클론항체(pAb)

ENO2 Rabbit pAb (A3118)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ENO2 Rabbit pAb (A3118)

Western blot analysis of various lysates, using ENO2 Rabbit pAb (A3118) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameENO2 Rabbit pAb
Catalog No.A3118
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human ENO2 (NP_001966.1).
SequenceMSIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERE
Gene ID
Swiss Prot
SynonymsNSE; HEL-S-279; ENO2
Calculated MW47kDa
Observed MW47kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, SH-SY5Y, Mouse brain, Mouse liver, Rat brain, Rat uterus
Cellular locationCell membrane, Cytoplasm.
Customer validation

IF(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - ENO2 Rabbit pAb (A3118)}

Western blot - ENO2 Rabbit pAb (A3118)

Western blot analysis of various lysates, using ENO2 Rabbit pAb (A3118) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A3118? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

ELISA 키트 (1)

재조합 단백질 (1)

제품 (2)

Secondary Antibodies (26)