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FOXO1/FOXO3A/FOXO4 Rabbit pAb (A17978)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using FOXO1/FOXO3A/FOXO4 Rabbit pAb (A17978) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - FOXO1/FOXO3A/FOXO4 Rabbit pAb (A17978)

Immunohistochemistry analysis of paraffin-embedded human placenta using FOXO1/FOXO3A/FOXO4 Rabbit pAb (A17978) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse liver using FOXO1/FOXO3A/FOXO4 Rabbit pAb (A17978) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat testis using FOXO1/FOXO3A/FOXO4 Rabbit pAb (A17978) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product name	FOXO1/FOXO3A/FOXO4 Rabbit pAb
Catalog No.	A17978
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The Forkhead family of transcription factors mediates many aspects of physiology, including stress response, metabolism, commitment to apoptosis, and development. The Forkhead box subfamily O (FoxO) proteins have garnered particular interest due to their involvement in the modulation of cancer, metabolism and cardiovascular biology.In mammals, four subfamily members have been identified: FOXO1 (previously also known as FKHR), FOXO3 (also known as FKHRL1), FOXO4 (also known as AFX), and FOXO6.

Immunogen information

Immunogen	A synthetic peptide of human FOXO1/FOXO3A/FOXO4 (NP_002006.2).
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW	80kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	293T, HeLa, Mouse liver, Mouse skeletal muscle, Mouse lung, Rat liver
Cellular location	cytoplasm, cytosol, mitochondrion, nucleoplasm, nucleus
Customer validation	WB(Mus musculus) IF(Mus musculus) WB(Mus musculus)