제품 > 항체 > 인산화 특이적 항체

Phospho-FOXO1-T24 + FOXO3A-T32 + FOXO4-T28 Rabbit mAb (AP1379)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from Jurkat cells, using Phospho-FOXO1-T24 + FOXO3A-T32 + FOXO4-T28 Rabbit mAb (AP1379) at 1:2000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-FOXO1-T24 + FOXO3A-T32 + FOXO4-T28 Rabbit mAb (AP1379) at 1:2000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Overview

Product name	Phospho-FOXO1-T24 + FOXO3A-T32 + FOXO4-T28 Rabbit mAb
Catalog No.	AP1379
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC57268

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T24 & T32 & T28 of human FOXO1/FOXO3A/FOXO4FOXO1.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	FoxO1/FoxO3a/FoxO4; Phospho-FOXO1-T24 + FOXO3A-T32 + FOXO4-T28
Calculated MW	70kDa/71kDa/48kDa/54kDa
Observed MW	78-82kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Jurkat treated by Calyculin A, NIH/3T3 treated by Calyculin A
Cellular location	Cytoplasm, Cytosol, Mitochondrion, Nucleoplasm, Nucleus.