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Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892) at 1:500 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)

Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product name	Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb
Catalog No.	AP0892
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T24 of human FOXO1/FOXO3A/FOXO4FOXO1 (NP_002006.2).
Sequence	SCTWP
Gene ID
Swiss Prot
Synonyms	FoxO1/FoxO3a/FoxO4; Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28
Calculated MW
Observed MW	65kDa/73kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Jurkat treated by Calyculin A, C6 treated by Calyculin A
Cellular location	cytoplasm, cytosol, mitochondrion, nucleoplasm, nucleus.