제품 > 항체 > 다중클론항체(pAb)

Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Fatty Acid Synthase (FASN) Rabbit pAb (A0461) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of various lysates, using Fatty Acid Synthase (FASN) Rabbit pAb (A0461) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

Western blot analysis of lysates from Rat liver, using Fatty Acid Synthase (FASN) Rabbit pAb (A0461) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

Western blot analysis of lysates from MCF7 cells, using Fatty Acid Synthase (FASN) Rabbit pAb (A0461) at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

Western blot analysis of various lysates, using Fatty Acid Synthase (FASN) Rabbit pAb (A0461) at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

Western blot analysis of lysates from wild type(WT) and Fatty Acid Synthase (FASN) Rabbit pAb knockout (KO) HeLa cells, using Fatty Acid Synthase (FASN) Rabbit pAb (A0461) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

Western blot analysis of lysates from wild type (WT) and Fatty Acid Synthase (FASN) knockout (KO) Hela(KO) cells, using Fatty Acid Synthase (FASN) (A0461) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Immunohistochemistry - Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Fatty Acid Synthase (FASN) Rabbit pAb (A0461) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Immunofluorescence analysis of HeLa cells using Fatty Acid Synthase (FASN) Rabbit pAb(A0461) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	Fatty Acid Synthase (FASN) Rabbit pAb
Catalog No.	A0461
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 2221-2511 of human Fatty Acid Synthase (FASN) (NP_004095.4).
Sequence	SLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG
Gene ID
Swiss Prot
Synonyms	FAS; OA-519; SDR27X1; Fatty Acid Synthase (FASN)
Calculated MW	273kDa
Observed MW

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	MCF7, A-549, HeLa, 293T, Mouse liver, Mouse thymus, Rat liver
Cellular location	Cytoplasm, Melanosome.
Customer validation	(Human fat cell, Breast cancer) WB(Mus musculus, Homo sapiens, Gallus gallus, Anas platyrhynchos, Mus musculus, Danio rerio) IHC(Homo sapiens)

Documents

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Fatty Acid Synthase (FASN) Rabbit pAb images

Western blot - Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Immunohistochemistry - Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Immunofluorescence - Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Nutrients

Application:

IF:

5.9

Species:

Mus musculus

PMID:

31991934

Title:

Sesamol Alleviates Obesity-Related Hepatic Steatosis via Activating Hepatic PKA Pathway

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Aging-US

Application:

IF:

3.9

Species:

Mus musculus

PMID:

33186123

Title:

The nuclear and cytoplasmic roles of miR-320 in non-alcoholic fatty liver disease.

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Nutrients

Application:

IF:

5.9

Species:

Homo sapiens

PMID:

34836271

Title:

Genistein Regulates Lipid Metabolism via Estrogen Receptor β and Its Downstream Signal Akt/mTOR in HepG2 Cells

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Front Med (Lausanne)

Application:

IF:

5.1

Species:

Mus musculus

PMID:

34881260

Title:

Pyruvate Might Bridge Gut Microbiota and Muscle Health in Aging Mice After Chronic High Dose of Leucine Supplementation

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Cell reports

Application:

IF:

7.5

Species:

Mus musculus

PMID:

35830798

Title:

RPA1 controls chromatin architecture and maintains lipid metabolic homeostasis

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Biochimica et biophysica acta. Molecular and cell biology of lipids

Application:

IF:

3.9

Species:

Homo sapiens

PMID:

36690321

Title:

Zeaxanthin prevents ferroptosis by promoting mitochondrial function and inhibiting the p53 pathway in free fatty acid-induced HepG2 cells

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Frontiers in cellular and infection microbiology

Application:

IF:

4.6

Species:

Homo sapiens

PMID:

36405969

Title:

Zika virus infection triggers lipophagy by stimulating the AMPK-ULK1 signaling in human hepatoma cells - PMC

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.7

Species:

Mus musculus

PMID:

36610148

Title:

Curcumin Treatment Suppresses Cachexia-Associated Adipose Wasting in Mice by Blocking the cAMP/PKA/CREB Signaling Pathway

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Nutrients

Application:

IF:

5.9

Species:

Mus musculus

PMID:

37111058

Title:

Hepatic G Protein-Coupled Receptor 180 Deficiency Ameliorates High Fat Diet-Induced Lipid Accumulation via the Gi-PKA-SREBP Pathway - PMC

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Scientific reports

Application:

IF:

3.8

Species:

Mus musculus

PMID:

37095192

Title:

Gut microbiota induces hepatic steatosis by modulating the T cells balance in high fructose diet mice - PMC

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Poultry science

Application:

IF:

4.4

Species:

Gallus gallus

PMID:

37748246

Title:

Transcriptome analysis of healthy and fatty liver revealed that inhibition of SLCO1B3 induces abnormal liver metabolism and lipid synthesis - PMC

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Acta pharmacologica Sinica

Application:

IF:

6.9

Species:

Homo sapiens

PMID:

37402997

Title:

6-Methyl flavone inhibits Nogo-B expression and improves high fructose diet-induced liver injury in mice

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.6

Species:

Homo sapiens

PMID:

38362150

Title:

Alpha-hederin reprograms multi-miRNAs activity and overcome small extracellular vesicles-mediated paclitaxel resistance in NSCLC - PMC

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

6.9

Species:

Homo sapiens

PMID:

38154274

Title:

Ergosterol peroxide blocks HDV infection as a novel entry inhibitor by targeting human NTCP receptor

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

PeerJ

Application:

IF:

2.3

Species:

Homo sapiens

PMID:

37692113

Title:

Chronic unpredictable mild stress promotes atherosclerosis via adipose tissue dysfunction in ApoE-/- mice - PMC

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Journal of animal science and biotechnology

Application:

IF:

6.3

Species:

Anas platyrhynchos

PMID:

35581591

Title:

Dietary methionine deficiency stunts growth and increases fat deposition via suppression of fatty acids transportation and hepatic catabolism in Pekin ducks

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

International journal of biological macromolecules

Application:

IF:

7.7

Species:

Homo sapiens, Mus musculus

PMID:

36473530

Title:

Hepatitis B virus X protein increases LASP1 SUMOylation to stabilize HER2 and facilitate hepatocarcinogenesis

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Journal of experimental & clinical cancer research : CR

Application:

WB, IHC

IF:

Species:

Homo sapiens

PMID:

36604718

Title:

Targeting fatty acid synthase modulates sensitivity of hepatocellular carcinoma to sorafenib via ferroptosis - PMC

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Fish & shellfish immunology

Application:

IF:

4.1

Species:

Danio rerio

PMID:

38272331

Title:

Low phosphorus increases hepatic lipid deposition, oxidative stress and inflammatory response via Acetyl-CoA carboxylase-dependent manner in zebrafish liver cells

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Nutrients

Application:

IF:

5.9

Species:

Mus musculus

PMID:

35334944

Title:

Liquiritigenin Inhibits Lipid Accumulation in 3T3-L1 Cells via mTOR-Mediated Regulation of the Autophagy Mechanism

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Cellular signalling

Application:

IF:

4.4

Species:

Homo sapiens

PMID:

37268162

Title:

HMGA2 knockdown alleviates the progression of nonalcoholic fatty liver disease (NAFLD) by downregulating SNAI2 expression

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

Molecular nutrition & food research

Application:

IF:

5.2

Species:

Mus musculus

PMID:

35297565

Title:

5-Heptadecylresorcinol Protects against Atherosclerosis in Apolipoprotein E-Deficient Mice by Modulating SIRT3 Signaling: The Possible Beneficial Effects of Whole Grain Consumption

References for Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Product:

Fatty Acid Synthase (FASN) Rabbit pAb

Journal:

iScience

Application:

IF:

5.8

Species:

Mus musculus

PMID:

38706871