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G6PD Rabbit mAb (A27296)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - G6PD Rabbit mAb (A27296)

Western blot analysis of various lysates using G6PD Rabbit mAb (A27296) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - G6PD Rabbit mAb (A27296)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using G6PD Rabbit mAb (A27296) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - G6PD Rabbit mAb (A27296)

Immunohistochemistry analysis of paraffin-embedded Rat thymus tissue using G6PD Rabbit mAb (A27296) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - G6PD Rabbit mAb (A27296)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using G6PD Rabbit mAb (A27296) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - G6PD Rabbit mAb (A27296)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using G6PD Rabbit mAb (A27296) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - G6PD Rabbit mAb (A27296)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using G6PD Rabbit mAb (A27296) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - G6PD Rabbit mAb (A27296)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using G6PD Rabbit mAb (A27296) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - G6PD Rabbit mAb (A27296)

Confocal imaging of HeLa cells using G6PD Rabbit mAb (A27296, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - G6PD Rabbit mAb (A27296)

Confocal imaging of C2C12 cells using G6PD Rabbit mAb (A27296, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameG6PD Rabbit mAb
Catalog No.A27296
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC67651
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 416-515 of human G6PD (NP_001035810.1).
SequenceEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTEADELMKRVGFQYEGTYKWVNPHKL
Gene ID
Swiss Prot
SynonymsG6PD1
Calculated MW60kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:5000 - 1:20000
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, NIH/3T3, C2C12, Mouse thymus
Cellular locationcytoplasm, cytoplasmic side of plasma membrane, cytosol, extracellular exosome, nucleus.

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ABclonal:Western blot - G6PD Rabbit mAb (A27296)}

Western blot - G6PD Rabbit mAb (A27296)

Western blot analysis of various lysates using G6PD Rabbit mAb (A27296) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - G6PD Rabbit mAb (A27296)}

Immunohistochemistry - G6PD Rabbit mAb (A27296)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using G6PD Rabbit mAb (A27296) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - G6PD Rabbit mAb (A27296)}

Immunohistochemistry - G6PD Rabbit mAb (A27296)

Immunohistochemistry analysis of paraffin-embedded Rat thymus tissue using G6PD Rabbit mAb (A27296) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - G6PD Rabbit mAb (A27296)}

Immunohistochemistry - G6PD Rabbit mAb (A27296)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using G6PD Rabbit mAb (A27296) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - G6PD Rabbit mAb (A27296)}

Immunohistochemistry - G6PD Rabbit mAb (A27296)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using G6PD Rabbit mAb (A27296) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - G6PD Rabbit mAb (A27296)}

Immunohistochemistry - G6PD Rabbit mAb (A27296)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using G6PD Rabbit mAb (A27296) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - G6PD Rabbit mAb (A27296)}

Immunohistochemistry - G6PD Rabbit mAb (A27296)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using G6PD Rabbit mAb (A27296) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - G6PD Rabbit mAb (A27296)}

Immunofluorescence - G6PD Rabbit mAb (A27296)

Confocal imaging of HeLa cells using G6PD Rabbit mAb (A27296, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - G6PD Rabbit mAb (A27296)}

Immunofluorescence - G6PD Rabbit mAb (A27296)

Confocal imaging of C2C12 cells using G6PD Rabbit mAb (A27296, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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