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G6PD Rabbit pAb (A1537)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - G6PD Rabbit pAb (A1537)

Western blot analysis of lysates from MCF7 cells, using G6PD Rabbit pAb (A1537) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - G6PD Rabbit pAb (A1537)

Western blot analysis of lysates from wild type (WT) and G6PD knockdown (KD) HeLa cells, using [KD Validated] G6PD Rabbit pAb (A1537) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - G6PD Rabbit pAb (A1537)

Western blot analysis of lysates from MCF7 cells, using [KD Validated] G6PD Rabbit pAb (A1537) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - G6PD Rabbit pAb (A1537)

Western blot analysis of lysates from C6 cells, using [KD Validated] G6PD Rabbit pAb (A1537) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - G6PD Rabbit pAb (A1537)

Western blot analysis of lysates from wild type(WT) and G6PD knockdown (KD) HeLa cells, using [KD Validated] G6PD Rabbit pAb (A1537) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - G6PD Rabbit pAb (A1537)

Immunofluorescence analysis of HeLa cells using G6PD Rabbit pAb (A1537) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameG6PD Rabbit pAb
Catalog No.A1537
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 195-515 of human G6PD (NP_001035810.1).
SequenceQIYRIDHYLGKEMVQNLMVLRFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTNSDDVRDEKVKVLKCISEVQANNVVLGQYVGNPDGEGEATKGYLDDPTVPRGSTTATFAAVVLYVENERWDGVPFILRCGKALNERKAEVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTEADELMKRVGFQYEGTYKWVNPHKL
Gene ID
Swiss Prot
SynonymsG6PD1; G6PD
Calculated MW59kDa
Observed MW59kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMCF7
Cellular locationcytoplasm, cytoplasmic side of plasma membrane, cytosol, extracellular exosome, nucleus.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - G6PD Rabbit pAb (A1537)}

Western blot - G6PD Rabbit pAb (A1537)

Western blot analysis of lysates from MCF7 cells, using G6PD Rabbit pAb (A1537) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - G6PD Rabbit pAb (A1537)}

Western blot - G6PD Rabbit pAb (A1537)

Western blot analysis of lysates from wild type (WT) and G6PD knockdown (KD) HeLa cells, using [KD Validated] G6PD Rabbit pAb (A1537) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - G6PD Rabbit pAb (A1537)}

Western blot - G6PD Rabbit pAb (A1537)

Western blot analysis of lysates from MCF7 cells, using [KD Validated] G6PD Rabbit pAb (A1537) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - G6PD Rabbit pAb (A1537)}

Western blot - G6PD Rabbit pAb (A1537)

Western blot analysis of lysates from C6 cells, using [KD Validated] G6PD Rabbit pAb (A1537) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - G6PD Rabbit pAb (A1537)}

Western blot - G6PD Rabbit pAb (A1537)

Western blot analysis of lysates from wild type(WT) and G6PD knockdown (KD) HeLa cells, using [KD Validated] G6PD Rabbit pAb (A1537) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - G6PD Rabbit pAb (A1537)}

Immunofluorescence - G6PD Rabbit pAb (A1537)

Immunofluorescence analysis of HeLa cells using G6PD Rabbit pAb (A1537) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1537? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

재조합 단백질 (1)

Secondary Antibodies (26)