제품 > 항체 > 다중클론항체(pAb)

GSK3α Rabbit pAb (A0645)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of extracts of various cell lines, using GSK3α antibody (A0645) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - GSK3α Rabbit pAb (A0645)

Immunofluorescence analysis of NIH/3T3 cells using GSK3α antibody (A0645) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product name	GSK3α Rabbit pAb
Catalog No.	A0645
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 409-483 of human GSK3α (NP_063937.2).
Sequence	LGTQLPNNRPLPPLFNFSAGELSIQPSLNAILIPPHLRSPAGTTTLTPSSQALTETPTSSDWQSTDATPTLTNSS
Gene ID
Swiss Prot
Synonyms	GSK3 alpha; GSK3A
Calculated MW	51kDa
Observed MW	51kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:20 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	U-87MG, A-431, HeLa, B-cell, Mouse liver
Cellular location	apical dendrite, axon, beta-catenin destruction complex, cytoplasm, cytosol, mitochondrion, nucleus, postsynapse, proximal dendrite
Customer validation	WB(Mus musculus, Homo sapiens)