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Phospho-GSK3α-S21 Rabbit pAb (AP0582)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from 293 and HeLa cells, using Phospho-GSK3α-S21 Rabbit pAb (AP0582) at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Western blot analysis of lysates from 293 cells, using Phospho-GSK3α-S21 Rabbit pAb (AP0582) at 1:1000 dilution or GSK3α antibody (A0645). 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

ABclonal:Immunoprecipitation - Phospho-GSK3α-S21 Rabbit pAb (AP0582)

Immunoprecipitation analysis of 200 μg extracts of 293T cells, using 3 μg Phospho-GSK3α-S21 pAb (AP0582). Western blot was performed from the immunoprecipitate using Phospho-GSK3α-S21 pAb (AP0582) at a dilution of 1:1000. 293T cells were treated by Insulin (100 nM) at 37℃ for 10 minutes after serum-starvation overnight.

Overview

Product name	Phospho-GSK3α-S21 Rabbit pAb
Catalog No.	AP0582
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S21 of human GSK3α (NP_063937.2).
Sequence	TSSFA
Gene ID
Swiss Prot
Synonyms	GSK3 alpha; GSK3A; Phospho-GSK3α-S21
Calculated MW	51kDa
Observed MW	51kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	293 treated by Insulin, HeLa treated by Insulin
Cellular location	apical dendrite, axon, beta-catenin destruction complex, cytoplasm, cytosol, mitochondrion, nucleus, postsynapse, proximal dendrite.
Customer validation	WB(Homo sapiens)