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Galectin 1/LGALS1 Rabbit mAb (A4732)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Galectin 1/LGALS1 Rabbit mAb (A4732) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - Galectin 1/LGALS1 Rabbit mAb (A4732)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Galectin 1/LGALS1 Rabbit mAb (A4732) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Galectin 1/LGALS1 Rabbit mAb (A4732)

Immunofluorescence analysis of NIH-3T3 cells using Galectin 1/LGALS1 Rabbit mAb (A4732) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Galectin 1/LGALS1 Rabbit mAb
Catalog No.	A4732
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1110

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Galectin 1/LGALS1 (P09382).
Sequence	MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVK
Gene ID
Swiss Prot
Synonyms	GBP; GAL1; Galectin 1/LGALS1
Calculated MW	15kDa
Observed MW	14kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	U2OS, A-549, HL-60, Mouse testis, Mouse thymus, Rat lung, Rat heart
Cellular location	Secreted, extracellular matrix, extracellular space
Customer validation	WB(Mus musculus)