Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from wild type (WT) and Galectin 1/Galectin 1/LGALS1 knockout (KO) 293T cells, using [KO Validated] Galectin 1/LGALS1 Rabbit pAb (A18040) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
Western blot analysis of lysates from SGC-7901 cells using Galectin 1/LGALS1 Rabbit pAb (A18040) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
| Product name | [KO Validated] Galectin 1/LGALS1 Rabbit pAb |
|---|---|
| Catalog No. | A18040 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human Galectin 1/Galectin 1/LGALS1 (NP_002296.1). |
|---|---|
| Sequence | MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD |
| Gene ID | |
| Swiss Prot | |
| Synonyms | GBP; GAL1; S1 |
| Calculated MW | 15kDa |
| Observed MW | 14kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | 293T |
| Cellular location | Secreted, extracellular matrix, extracellular space. |
| Customer validation | WB(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A18040? Please let us know so that we can cite the reference in this datasheet.
![ABclonal:Western blot - [KO Validated] Galectin 1/LGALS1 Rabbit pAb (A18040)](https://img.abclonal.com/abclonal-manage/Catalog/A18040/A18040_1.jpg?t=1725334830 "ABclonal:Western blot - [KO Validated] Galectin 1/LGALS1 Rabbit pAb (A18040)")
![ABclonal:Western blot - [KO Validated] Galectin 1/LGALS1 Rabbit pAb (A18040)](https://img.abclonal.com/abclonal-manage/Catalog/A18040/A18040_2.jpg?t=1725334830 "ABclonal:Western blot - [KO Validated] Galectin 1/LGALS1 Rabbit pAb (A18040)")
