제품 > 항체 > 다중클론항체(pAb)

Galectin 1/LGALS1 Rabbit pAb (A1580)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using Galectin 1/LGALS1 antibody (A1580) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Galectin 1/LGALS1 Rabbit pAb (A1580)

Immunofluorescence analysis of NIH/3T3 cells using Galectin 1/LGALS1 Rabbit pAb (A1580) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using Galectin 1/LGALS1 Rabbit pAb (A1580) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using Galectin 1/LGALS1 Rabbit pAb (A1580) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	Galectin 1/LGALS1 Rabbit pAb
Catalog No.	A1580
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human Galectin 1/Galectin 1/LGALS1 (NP_002296.1).
Sequence	MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD
Gene ID
Swiss Prot
Synonyms	GBP; GAL1
Calculated MW	15kDa
Observed MW	14kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	U2OS, A-549, Mouse testis, Mouse thymus, Rat lung, Rat heart
Cellular location	Secreted, extracellular matrix, extracellular space
Customer validation	IHC(Homo sapiens) WB(Mus musculus)

Documents

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Research Area

Galectin 1/LGALS1 Rabbit pAb images

Western blot - Galectin 1/LGALS1 Rabbit pAb (A1580)

Immunofluorescence - Galectin 1/LGALS1 Rabbit pAb (A1580)

Immunofluorescence analysis of NIH/3T3 cells using Galectin 1/LGALS1 Rabbit pAb (A1580) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - Galectin 1/LGALS1 Rabbit pAb (A1580)

Immunofluorescence analysis of PC-12 cells using Galectin 1/LGALS1 Rabbit pAb (A1580) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - Galectin 1/LGALS1 Rabbit pAb (A1580)

Immunofluorescence analysis of U2OS cells using Galectin 1/LGALS1 Rabbit pAb (A1580) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.