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HDAC6 Rabbit pAb (A11259)

Publications (10) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HDAC6 Rabbit pAb (A11259)

Western blot analysis of lysates from Rat liver, using HDAC6 Rabbit pAb (A11259) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - HDAC6 Rabbit pAb (A11259)

Immunohistochemistry analysis of paraffin-embedded Human liver using HDAC6 Rabbit pAb (A11259) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - HDAC6 Rabbit pAb (A11259)

Immunofluorescence analysis of HeLa cells using HDAC6 Rabbit pAb (A11259) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HDAC6 Rabbit pAb (A11259)

Immunofluorescence analysis of L929 cells using HDAC6 Rabbit pAb(A11259) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - HDAC6 Rabbit pAb (A11259)

Immunoprecipitation analysis of 100 μg extracts of HepG2 cells using 3 μg HDAC6 antibody (A11259). Western blot was performed from the immunoprecipitate using HDAC6 antibody (A11259) at a dilution of 1:1000.

Overview

Product nameHDAC6 Rabbit pAb
Catalog No.A11259
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 836-1104 of human HDAC6 (NP_006035.2).
SequenceVMKVEDREGPSSSKLVTKKAPQPAKPRLAERMTTREKKVLEAGMGKVTSASFGEESTPGQTNSETAVVALTQDQPSEAATGGATLAQTISEAAIGGAMLGQTTSEEAVGGATPDQTTSEETVGGAILDQTTSEDAVGGATLGQTTSEEAVGGATLAQTTSEAAMEGATLDQTTSEEAPGGTELIQTPLASSTDHQTPPTSPVQGTTPQISPSTLIGSLRTLELGSESQGASESQAPGEENLLGEAAGGQDMADSMLMQGSRGLTDQAIF
Gene ID
Swiss Prot
SynonymsHD6; JM21; CPBHM; PPP1R90; HDAC6
Calculated MW131kDa
Observed MW160kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesRat liver
Cellular locationCell projection, Cytoplasm, Nucleus, Perikaryon, axon, dendrite.
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

IHC(Rattus norvegicus, Mus musculus)

IP(Mus musculus)

IF(Rattus norvegicus, Mus musculus, Homo sapiens)

IHC(Homo sapiens)

IP(Homo sapiens)

PLA(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - HDAC6 Rabbit pAb (A11259)}

Western blot - HDAC6 Rabbit pAb (A11259)

Western blot analysis of lysates from Rat liver, using HDAC6 Rabbit pAb (A11259) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - HDAC6 Rabbit pAb (A11259)}

Immunohistochemistry - HDAC6 Rabbit pAb (A11259)

Immunohistochemistry analysis of paraffin-embedded Human liver using HDAC6 Rabbit pAb (A11259) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - HDAC6 Rabbit pAb (A11259)}

Immunofluorescence - HDAC6 Rabbit pAb (A11259)

Immunofluorescence analysis of HeLa cells using HDAC6 Rabbit pAb (A11259) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HDAC6 Rabbit pAb (A11259)}

Immunofluorescence - HDAC6 Rabbit pAb (A11259)

Immunofluorescence analysis of L929 cells using HDAC6 Rabbit pAb(A11259) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - HDAC6 Rabbit pAb (A11259)}

Immunoprecipitation - HDAC6 Rabbit pAb (A11259)

Immunoprecipitation analysis of 100 μg extracts of HepG2 cells using 3 μg HDAC6 antibody (A11259). Western blot was performed from the immunoprecipitate using HDAC6 antibody (A11259) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)