제품 > 항체 > 단일클론항체(mAb)

HP1 gamma/CBX3 Rabbit mAb (A4283)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HP1 gamma/CBX3 Rabbit mAb (A4283)

Western blot analysis of various lysates using HP1 gamma/CBX3 Rabbit mAb (A4283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - HP1 gamma/CBX3 Rabbit mAb (A4283)

Western blot analysis of lysates from Rat brain, using HP1 gamma/CBX3 Rabbit mAb (A4283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Immunohistochemistry - HP1 gamma/CBX3 Rabbit mAb (A4283)

Immunohistochemistry analysis of paraffin-embedded human colon using HP1 gamma/CBX3 Rabbit mAb (A4283) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameHP1 gamma/CBX3 Rabbit mAb
Catalog No.A4283
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0952
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human HP1 gamma/CBX3 (NP_057671.2).
SequenceMASNKTTLQKMGKKQNGKSKKVEEAEPEEFVVEKVLDRRVVNGKVEYFLKWKGFTDADNTWEPEENLDCPELIEAFLNSQKAGKEKDGTKRKSLSDSESD
Gene ID
Swiss Prot
SynonymsHECH; HP1-GAMMA; HP1Hs-gamma; HP1 gamma/CBX3
Calculated MW21kDa
Observed MW22kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, SH-SY5Y, Mouse spleen, Mouse thymus, Rat brain
Cellular locationNucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - HP1 gamma/CBX3 Rabbit mAb (A4283)}

Western blot - HP1 gamma/CBX3 Rabbit mAb (A4283)

Western blot analysis of various lysates using HP1 gamma/CBX3 Rabbit mAb (A4283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - HP1 gamma/CBX3 Rabbit mAb (A4283)}

Western blot - HP1 gamma/CBX3 Rabbit mAb (A4283)

Western blot analysis of lysates from Rat brain, using HP1 gamma/CBX3 Rabbit mAb (A4283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Immunohistochemistry - HP1 gamma/CBX3 Rabbit mAb (A4283)}

Immunohistochemistry - HP1 gamma/CBX3 Rabbit mAb (A4283)

Immunohistochemistry analysis of paraffin-embedded human colon using HP1 gamma/CBX3 Rabbit mAb (A4283) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (4)

Secondary Antibodies (26)