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Human IgG3 Rabbit mAb (A19713)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Human IgG3 Rabbit mAb (A19713)

Western blot analysis of lysates from human plasma, using Human IgG3 Rabbit mAb (A19713) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameHuman IgG3 Rabbit mAb
Catalog No.A19713
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2243
Predicted to enable antigen binding activity and immunoglobulin receptor binding activity. Involved in retina homeostasis. Located in blood microparticle and extracellular exosome.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human IgG3 (P01860).
SequenceVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYTCNVNHKPSNTKVDKRVELKTPLGDTTHTCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPEPKSC
Gene ID
Swiss Prot
SynonymsIgG3; Human IgG3
Calculated MW41kDa
Observed MW60kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive sampleshuman plasma
Cellular locationBlood microparticle, External side of plasma membrane, Extracellular exosome, Extracellular region, Extracellular space, Immunoglobulin complex, Circulating

Documents

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Lot number

    ABclonal:Western blot - Human IgG3 Rabbit mAb (A19713)}

    Western blot - Human IgG3 Rabbit mAb (A19713)

    Western blot analysis of lysates from human plasma, using Human IgG3 Rabbit mAb (A19713) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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