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ICAM3 / CD50 Rabbit mAb (A20877)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - ICAM3 / CD50 Rabbit mAb (A20877)

Western blot analysis of various lysates using ICAM3 / CD50 Rabbit mAb (A20877) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ICAM3 / CD50 Rabbit mAb (A20877)

Immunohistochemistry analysis of paraffin-embedded human liver using ICAM3 / CD50 Rabbit mAb (A20877) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ICAM3 / CD50 Rabbit mAb (A20877)

Immunohistochemistry analysis of paraffin-embedded human tonsil using ICAM3 / CD50 Rabbit mAb (A20877) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameICAM3 / CD50 Rabbit mAb
Catalog No.A20877
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2822
The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human ICAM3 / CD50 (P32942).
SequenceCNGSQITGSSNITVYRLPERVELAPLPPWQPVGQNFTLRCQVEDGSPRTSLTVVLLRWEEELSRQPAVEEPAEVTATVLASRDDHGAPFSCRTELDMQPQG
Gene ID
Swiss Prot
SynonymsCD50; CDW50; ICAM-R; ICAM3 / CD50
Calculated MW60kDa
Observed MW120-140kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-937, Jurkat
Cellular locationMembrane, Single-pass type I membrane protein

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ABclonal:Western blot - ICAM3 / CD50 Rabbit mAb (A20877)}

Western blot - ICAM3 / CD50 Rabbit mAb (A20877)

Western blot analysis of various lysates using ICAM3 / CD50 Rabbit mAb (A20877) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - ICAM3 / CD50 Rabbit mAb (A20877)}

Immunohistochemistry - ICAM3 / CD50 Rabbit mAb (A20877)

Immunohistochemistry analysis of paraffin-embedded human liver using ICAM3 / CD50 Rabbit mAb (A20877) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ICAM3 / CD50 Rabbit mAb (A20877)}

Immunohistochemistry - ICAM3 / CD50 Rabbit mAb (A20877)

Immunohistochemistry analysis of paraffin-embedded human tonsil using ICAM3 / CD50 Rabbit mAb (A20877) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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