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IFNGR1 Rabbit pAb (A5748)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IFNGR1 Rabbit pAb (A5748)

Western blot analysis of various lysates using IFNGR1 Rabbit pAb (A5748) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - IFNGR1 Rabbit pAb (A5748)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using IFNGR1 Rabbit pAb (A5748) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - IFNGR1 Rabbit pAb (A5748)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using IFNGR1 Rabbit pAb (A5748) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product nameIFNGR1 Rabbit pAb
Catalog No.A5748
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 270-489 of human IFNGR1 (NP_000407.1).
SequenceIKKINPLKEKSIILPKSLISVVRSATLETKPESKYVSLITSYQPFSLEKEVVCEEPLSPATVPGMHTEDNPGKVEHTEELSSITEVVTTEENIPDVVPGSHLTPIERESSSPLSSNQSEPGSIALNSYHSRNCSESDHSRNGFDTDSSCLESHSSLSDSEFPPNNKGEIKTEGQELITVIKAPTSFGYDKPHVLVDLLVDDSGKESLIGYRPTEDSKEFS
Gene ID
Swiss Prot
SynonymsCD119; IFNGR; IMD27A; IMD27B; IFNGR1
Calculated MW54kDa
Observed MW80-90kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, Mouse liver, Rat liver
Cellular locationMembrane, Single-pass type I membrane protein.
Customer validation

IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - IFNGR1 Rabbit pAb (A5748)}

Western blot - IFNGR1 Rabbit pAb (A5748)

Western blot analysis of various lysates using IFNGR1 Rabbit pAb (A5748) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - IFNGR1 Rabbit pAb (A5748)}

Immunohistochemistry - IFNGR1 Rabbit pAb (A5748)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using IFNGR1 Rabbit pAb (A5748) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - IFNGR1 Rabbit pAb (A5748)}

Immunohistochemistry - IFNGR1 Rabbit pAb (A5748)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using IFNGR1 Rabbit pAb (A5748) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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ELISA 키트 (1)

재조합 단백질 (1)

항체 (5)

Secondary Antibodies (26)