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IGF2BP1/IMP1 Rabbit pAb (A1517)

Reviews (2)Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IGF2BP1/IMP1 Rabbit pAb (A1517)

Western blot analysis of various lysates using IGF2BP1/IMP1 Rabbit pAb (A1517) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - IGF2BP1/IMP1 Rabbit pAb (A1517)

Immunohistochemistry analysis of paraffin-embedded human stomach using IGF2BP1/IMP1 Rabbit pAb (A1517) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - IGF2BP1/IMP1 Rabbit pAb (A1517)

Immunofluorescence analysis of U2OS cells using IGF2BP1/IMP1 Rabbit pAb (A1517) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for IGF2BP1/IMP1 Rabbit pAb(A1517)
Review
ABclonal: review for IGF2BP1/IMP1 Rabbit pAb(A1517)

Overview

Product nameIGF2BP1/IMP1 Rabbit pAb
Catalog No.A1517
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 478-577 of human IGF2BP1/IMP1 (NP_006537.3).
SequenceNFFGPKEEVKLETHIRVPASAAGRVIGKGGKTVNELQNLTAAEVVVPRDQTPDENDQVIVKIIGHFYASQMAQRKIRDILAQVKQQHQKGQSNQAQARRK
Gene ID
Swiss Prot
SynonymsIMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1; IGF2BP1/IMP1
Calculated MW63kDa
Observed MW71kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3, HeLa, A-549, Jurkat, Mouse testis, Mouse kidney, Rat testis
Cellular locationCell projection, Cytoplasm, Nucleus, axon, dendrite, dendritic spine, filopodium, growth cone, lamellipodium, perinuclear region
Customer validation

(Huh7.5.1,HepG2, Human cells)

RIP(Homo sapiens)

WB(Homo sapiens, Mus musculus)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - IGF2BP1/IMP1 Rabbit pAb (A1517)}

Western blot - IGF2BP1/IMP1 Rabbit pAb (A1517)

Western blot analysis of various lysates using IGF2BP1/IMP1 Rabbit pAb (A1517) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - IGF2BP1/IMP1 Rabbit pAb (A1517)}

Immunohistochemistry - IGF2BP1/IMP1 Rabbit pAb (A1517)

Immunohistochemistry analysis of paraffin-embedded human stomach using IGF2BP1/IMP1 Rabbit pAb (A1517) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - IGF2BP1/IMP1 Rabbit pAb (A1517)}

Immunofluorescence - IGF2BP1/IMP1 Rabbit pAb (A1517)

Immunofluorescence analysis of U2OS cells using IGF2BP1/IMP1 Rabbit pAb (A1517) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (4)

Secondary Antibodies (26)