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IGF2BP2/IMP2 Rabbit pAb (A2749)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - IGF2BP2/IMP2 Rabbit pAb (A2749)

Western blot analysis of various lysates using IGF2BP2/IMP2 Rabbit pAb (A2749) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - IGF2BP2/IMP2 Rabbit pAb (A2749)

Immunohistochemistry analysis of paraffin-embedded mouse liver using IGF2BP2/IMP2 Rabbit pAb (A2749) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - IGF2BP2/IMP2 Rabbit pAb (A2749)

Immunofluorescence analysis of U2OS cells using IGF2BP2/IMP2 Rabbit pAb (A2749) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameIGF2BP2/IMP2 Rabbit pAb
Catalog No.A2749
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that binds the 5' UTR of insulin-like growth factor 2 (IGF2) mRNA and regulates its translation. It plays an important role in metabolism and variation in this gene is associated with susceptibility to diabetes. Alternative splicing and promoter usage results in multiple transcript variants. Related pseudogenes are found on several chromosomes.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 500-599 of human IGF2BP2/IMP2 (NP_006539.3).
SequenceNFFNPKEEVKLEAHIRVPSSTAGRVIGKGGKTVNELQNLTSAEVIVPRDQTPDENEEVIVRIIGHFFASQTAQRKIREIVQQVKQQEQKYPQGVASQRSK
Gene ID
Swiss Prot
SynonymsIMP2; IMP-2; VICKZ2; IGF2BP2/IMP2
Calculated MW66kDa
Observed MW66kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, Mouse ovary
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - IGF2BP2/IMP2 Rabbit pAb (A2749)}

Western blot - IGF2BP2/IMP2 Rabbit pAb (A2749)

Western blot analysis of various lysates using IGF2BP2/IMP2 Rabbit pAb (A2749) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - IGF2BP2/IMP2 Rabbit pAb (A2749)}

Immunohistochemistry - IGF2BP2/IMP2 Rabbit pAb (A2749)

Immunohistochemistry analysis of paraffin-embedded mouse liver using IGF2BP2/IMP2 Rabbit pAb (A2749) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - IGF2BP2/IMP2 Rabbit pAb (A2749)}

Immunofluorescence - IGF2BP2/IMP2 Rabbit pAb (A2749)

Immunofluorescence analysis of U2OS cells using IGF2BP2/IMP2 Rabbit pAb (A2749) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A2749? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

재조합 단백질 (1)

Secondary Antibodies (26)