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[KD Validated] CDX2 Rabbit mAb (A19030)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] CDX2 Rabbit mAb (A19030)

Western blot analysis of various lysates using [KD Validated] CDX2 Rabbit mAb (A19030) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Western blot - [KD Validated] CDX2 Rabbit mAb (A19030)

Western blot analysis of lysates from wild type(WT) and CDX2 knockdown (KD) 293T cells, using [KD Validated] CDX2 Rabbit mAb (A19030) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KD Validated] CDX2 Rabbit mAb (A19030)

Immunohistochemistry analysis of paraffin-embedded Human appendix using [KD Validated] CDX2 Rabbit mAb (A19030) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] CDX2 Rabbit mAb (A19030)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KD Validated] CDX2 Rabbit mAb (A19030) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] CDX2 Rabbit mAb (A19030)

Confocal imaging of Human colon using [KD Validated] CDX2 Rabbit mAb (A19030, dilution 1:100)(Red). DAPI was used for nuclear staining (blue). Objective: 40x.

ABclonal:Immunoprecipitation - [KD Validated] CDX2 Rabbit mAb (A19030)

Immunoprecipitation analysis of 600 μg extracts of 293T cells using 3 μg [KD Validated] CDX2 Rabbit mAb (A19030). Western blot was performed from the immunoprecipitate using CDX2 antibody (A19030) at a dilution of 1:500.

Overview

Product name[KD Validated] CDX2 Rabbit mAb
Catalog No.A19030
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0450
This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human CDX2 (Q99626).
SequenceMYVSYLLDKDVSMYPSSVRHSGGLNLAPQNFVSPPQYPDYGGYHVAAAAAAAANLDSAQSPGPSWPAAYGAPLREDWNGYAPGGAAAAANAVAHGLNGGS
Gene ID
Swiss Prot
SynonymsCDX3; CDX-3; CDX2/AS; X2
Calculated MW34kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:500 - 1:2000
  • IF/ICC 1:100 - 1:1000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesSW480, 293T
Cellular locationNucleus.
Customer validation

IF(Mus musculus)

IHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KD Validated] CDX2 Rabbit mAb (A19030)}

Western blot - [KD Validated] CDX2 Rabbit mAb (A19030)

Western blot analysis of various lysates using [KD Validated] CDX2 Rabbit mAb (A19030) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Western blot - [KD Validated] CDX2 Rabbit mAb (A19030)}

Western blot - [KD Validated] CDX2 Rabbit mAb (A19030)

Western blot analysis of lysates from wild type(WT) and CDX2 knockdown (KD) 293T cells, using [KD Validated] CDX2 Rabbit mAb (A19030) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KD Validated] CDX2 Rabbit mAb (A19030)}

Immunohistochemistry - [KD Validated] CDX2 Rabbit mAb (A19030)

Immunohistochemistry analysis of paraffin-embedded Human appendix using [KD Validated] CDX2 Rabbit mAb (A19030) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] CDX2 Rabbit mAb (A19030)}

Immunohistochemistry - [KD Validated] CDX2 Rabbit mAb (A19030)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KD Validated] CDX2 Rabbit mAb (A19030) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KD Validated] CDX2 Rabbit mAb (A19030)}

Immunofluorescence - [KD Validated] CDX2 Rabbit mAb (A19030)

Confocal imaging of Human colon using [KD Validated] CDX2 Rabbit mAb (A19030, dilution 1:100)(Red). DAPI was used for nuclear staining (blue). Objective: 40x.
ABclonal:Immunoprecipitation - [KD Validated] CDX2 Rabbit mAb (A19030)}

Immunoprecipitation - [KD Validated] CDX2 Rabbit mAb (A19030)

Immunoprecipitation analysis of 600 μg extracts of 293T cells using 3 μg [KD Validated] CDX2 Rabbit mAb (A19030). Western blot was performed from the immunoprecipitate using CDX2 antibody (A19030) at a dilution of 1:500.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A19030? Please let us know so that we can cite the reference in this datasheet.

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Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)