제품 > 항체 > 단일클론항체(mAb)

[KD Validated] CDX2 Rabbit mAb (A20222)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - [KD Validated] CDX2 Rabbit mAb (A20222)

Western blot analysis of lysates from SW620 cells, using [KD Validated] CDX2 Rabbit mAb (A20222) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KD Validated] CDX2 Rabbit mAb (A20222)

Western blot analysis of lysates from wild type(WT) and CDX2 knockdown(KD) 293T cells, using [KD Validated] CDX2 Rabbit mAb (A20222) at1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KD Validated] CDX2 Rabbit mAb (A20222)

Western blot analysis of lysates from Mouse colon, using [KD Validated] CDX2 Rabbit mAb (A20222) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name[KD Validated] CDX2 Rabbit mAb
Catalog No.A20222
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC3070
This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human CDX2 (Q99626 ).
SequenceMYVSYLLDKDVSMYPSSVRHSGGLNLAPQNFVSPPQYPDYGGYHVAAAAAAAANLDSAQSPGPSWPAAYGAPLREDWNGYAPGGAAAAANAVAHGLNGGS
Gene ID
Swiss Prot
SynonymsCDX3; CDX-3; CDX2/AS; X2
Calculated MW34kDa
Observed MW38kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSW620, 293T, Mouse colon
Cellular locationNucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KD Validated] CDX2 Rabbit mAb (A20222)}

Western blot - [KD Validated] CDX2 Rabbit mAb (A20222)

Western blot analysis of lysates from SW620 cells, using [KD Validated] CDX2 Rabbit mAb (A20222) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KD Validated] CDX2 Rabbit mAb (A20222)}

Western blot - [KD Validated] CDX2 Rabbit mAb (A20222)

Western blot analysis of lysates from wild type(WT) and CDX2 knockdown(KD) 293T cells, using [KD Validated] CDX2 Rabbit mAb (A20222) at1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KD Validated] CDX2 Rabbit mAb (A20222)}

Western blot - [KD Validated] CDX2 Rabbit mAb (A20222)

Western blot analysis of lysates from Mouse colon, using [KD Validated] CDX2 Rabbit mAb (A20222) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

Secondary Antibodies (26)