제품 > 항체 > 다중클론항체(pAb)

[KD Validated] ERK1 Rabbit pAb (A0228)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using [KD Validated] ERK1 Rabbit pAb (A0228) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of lysates from wild type (WT) and ERK1 knockdown (KD) 293T cells, using [KD Validated] ERK1 Rabbit pAb (A0228) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KD Validated] ERK1 Rabbit pAb (A0228)

Immunohistochemistry analysis of paraffin-embedded rat lung using [KD Validated] ERK1 Rabbit pAb (A0228) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human kidney using [KD Validated] ERK1 Rabbit pAb (A0228) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse heart using [KD Validated] ERK1 Rabbit pAb (A0228) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product name	[KD Validated] ERK1 Rabbit pAb
Catalog No.	A0228
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human ERK1 (NP_002737.2).
Sequence	MAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENV
Gene ID
Swiss Prot
Synonyms	ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; K1
Calculated MW	43kDa
Observed MW	40kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	DU145, HeLa, 293T
Cellular location	Cytoplasm, Nucleus
Customer validation	WB(Homo sapiens, Mus musculus, Pseudomonas syringae)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KD Validated] ERK1 Rabbit pAb images

Western blot - [KD Validated] ERK1 Rabbit pAb (A0228)

Immunohistochemistry - [KD Validated] ERK1 Rabbit pAb (A0228)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KD Validated] ERK1 Rabbit pAb (A0228)

Product:

[KD Validated] ERK1 Rabbit pAb

Journal:

Scientific Reports

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

26931521

Title:

Transcriptional regulation of miR-15b by c-Rel and CREB in Japanese encephalitis virus infection

References for [KD Validated] ERK1 Rabbit pAb (A0228)

Product:

[KD Validated] ERK1 Rabbit pAb

Journal:

International Journal of Clinical and Experimental Pathology

Application:

IF:

1.1

Species:

Homo sapiens

PMID:

24427333

Title:

Activation of AKT/ERK confers non-small cell lung cancer cells resistance to vinorelbine

References for [KD Validated] ERK1 Rabbit pAb (A0228)

Product:

[KD Validated] ERK1 Rabbit pAb

Journal:

International Journal Of Clinical and Experimental Pathology

Application:

IF:

1.1

Species:

Homo sapiens

PMID:

24427328

Title:

KAT5 silencing induces apoptosis of GBC-SD cells through p38MAPK-mediated upregulation of cleaved Casp9

References for [KD Validated] ERK1 Rabbit pAb (A0228)

Product:

[KD Validated] ERK1 Rabbit pAb

Journal:

International Journal Of Clinical and Experimental Pathology

Application:

IF:

1.1

Species:

Homo sapiens

PMID:

23923067

Title:

RAF1-MEK1-ERK/AKT axis may confer NSCLC cell lines resistance to erlotinib

References for [KD Validated] ERK1 Rabbit pAb (A0228)

Product:

[KD Validated] ERK1 Rabbit pAb

Journal:

International Journal Of Clinical and Experimental Pathology

Application:

IF:

1.1

Species:

Homo sapiens

PMID:

24696725

Title:

RNAi screening with shRNAs against histone methylation-related genes reveals determinants of sorafenib sensitivity in hepatocellular carcinoma cells

References for [KD Validated] ERK1 Rabbit pAb (A0228)

Product:

[KD Validated] ERK1 Rabbit pAb

Journal:

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

Application:

IF:

4.6

Species:

Homo sapiens

PMID:

28680855

Title:

p21-Activated Kinase 4 Signaling Promotes Japanese Encephalitis Virus-Mediated Inflammation in Astrocytes

References for [KD Validated] ERK1 Rabbit pAb (A0228)

Product:

[KD Validated] ERK1 Rabbit pAb

Journal:

Journal Of Ethnopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

25978950

Title:

Effect of Fructus Ligustri Lucidi on osteoblastic like cell-line MC3T3-E1

References for [KD Validated] ERK1 Rabbit pAb (A0228)

Product:

[KD Validated] ERK1 Rabbit pAb

Journal:

International Journal Of Clinical and Experimental Pathology

Application:

IF:

1.1

Species:

Homo sapiens

PMID:

24817940

Title:

AKT/ERK activation is associated with gastric cancer cell resistance to paclitaxel

References for [KD Validated] ERK1 Rabbit pAb (A0228)

Product:

[KD Validated] ERK1 Rabbit pAb

Journal:

Brain research bulletin

Application:

IF:

3.5

Species:

Homo sapiens

PMID:

37149267

Title:

Else_BRB_110660Docosahexaenoic acid promotes M2 microglia phenotype via activating PPARγ-mediated ERK/AKT pathway against cerebral ischemia …

References for [KD Validated] ERK1 Rabbit pAb (A0228)

Product:

[KD Validated] ERK1 Rabbit pAb

Journal:

Plants (Basel, Switzerland)

Application:

IF:

4.5

Species:

Pseudomonas syringae

PMID:

37571011

Title:

Nitrile-Specific Protein NSP2 and Its Interacting Protein MPK3 Synergistically Regulate Plant Disease Resistance in Arabidopsis - PMC

References for [KD Validated] ERK1 Rabbit pAb (A0228)

Product:

[KD Validated] ERK1 Rabbit pAb

Journal:

Frontiers in microbiology

Application:

IF:

6.1

Species:

Mus musculus

PMID:

35847084

Title:

EGFR Activation Impairs Antiviral Activity of Interferon Signaling in Brain Microvascular Endothelial Cells During Japanese Encephalitis Virus Infection - PMC

References for [KD Validated] ERK1 Rabbit pAb (A0228)

Product:

[KD Validated] ERK1 Rabbit pAb

Journal:

eLife

Application:

IF:

6.4

Species:

Homo sapiens,Mus musculus

PMID:

35975983