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Phospho-ERK1-Y204 Rabbit pAb (AP0235)

Publications (10) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-ERK1-Y204 Rabbit pAb (AP0235)

Western blot analysis of lysates from NIH/3T3 cells using Phospho-ERK1-Y204 Rabbit pAb (AP0235) at 1:1000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Phospho-ERK1-Y204 Rabbit pAb (AP0235)

Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-ERK1-Y204 Rabbit pAb (AP0235) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-ERK1-Y204 Rabbit pAb (AP0235)

Immunohistochemistry analysis of paraffin-embedded Human uterus using Phospho-ERK1-Y204 Rabbit pAb (AP0235) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-ERK1-Y204 Rabbit pAb (AP0235)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using Phospho-ERK1-Y204 Rabbit pAb (AP0235) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-ERK1-Y204 Rabbit pAb (AP0235)

Immunofluorescence analysis of methanol-fixed HeLa cells showing centrosome and nuclear staining using Phospho-ERK1-Y204 Rabbit pAb (AP0235).

ABclonal:Immunoprecipitation - Phospho-ERK1-Y204 Rabbit pAb (AP0235)

Immunoprecipitation analysis of 200 μg extracts of Jurkat cells, using 3 μg Phospho-ERK1-Y204 Rabbit pAb (AP0235). Western blot was performed from the immunoprecipitate using Phospho-ERK1-Y204 Rabbit pAb (AP0235) at a dilution of 1:1000. Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.

Overview

Product namePhospho-ERK1-Y204 Rabbit pAb
Catalog No.AP0235
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
ImmunogenA synthetic phosphorylated peptide around Y204 of human ERK1 (NP_002737.2).
SequenceTEYVA
Gene ID
Swiss Prot
SynonymsERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; Phospho-ERK1-Y204
Calculated MW43kDa
Observed MW42kDa/44kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:100 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesNIH/3T3 treated by PMA
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa, Gallus gallus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Phospho-ERK1-Y204 Rabbit pAb (AP0235)}

Western blot - Phospho-ERK1-Y204 Rabbit pAb (AP0235)

Western blot analysis of lysates from NIH/3T3 cells using Phospho-ERK1-Y204 Rabbit pAb (AP0235) at 1:1000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Phospho-ERK1-Y204 Rabbit pAb (AP0235)}

Immunohistochemistry - Phospho-ERK1-Y204 Rabbit pAb (AP0235)

Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-ERK1-Y204 Rabbit pAb (AP0235) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-ERK1-Y204 Rabbit pAb (AP0235)}

Immunohistochemistry - Phospho-ERK1-Y204 Rabbit pAb (AP0235)

Immunohistochemistry analysis of paraffin-embedded Human uterus using Phospho-ERK1-Y204 Rabbit pAb (AP0235) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-ERK1-Y204 Rabbit pAb (AP0235)}

Immunohistochemistry - Phospho-ERK1-Y204 Rabbit pAb (AP0235)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using Phospho-ERK1-Y204 Rabbit pAb (AP0235) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - Phospho-ERK1-Y204 Rabbit pAb (AP0235)}

Immunofluorescence - Phospho-ERK1-Y204 Rabbit pAb (AP0235)

Immunofluorescence analysis of methanol-fixed HeLa cells showing centrosome and nuclear staining using Phospho-ERK1-Y204 Rabbit pAb (AP0235).
ABclonal:Immunoprecipitation - Phospho-ERK1-Y204 Rabbit pAb (AP0235)}

Immunoprecipitation - Phospho-ERK1-Y204 Rabbit pAb (AP0235)

Immunoprecipitation analysis of 200 μg extracts of Jurkat cells, using 3 μg Phospho-ERK1-Y204 Rabbit pAb (AP0235). Western blot was performed from the immunoprecipitate using Phospho-ERK1-Y204 Rabbit pAb (AP0235) at a dilution of 1:1000. Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AP0235? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

[KD Validated] ERK1 Rabbit pAb
A0228

ELISAWBIHC-PIF/ICC

Reactivity:Human, Mouse, Rat

[KD Validated] ERK1 Rabbit pAb

[KD Validated] ERK1 Rabbit mAb
A19561

WBIHC-PIF/ICCIPELISA

Reactivity:Human, Mouse, Rat

[KD Validated] ERK1 Rabbit mAb

ERK1 Rabbit mAb
A21975

ELISAWBIF/ICC

Reactivity:Rat

ERK1 Rabbit mAb

ELISA 키트 (1)

Human Mitogen-activated protein kinase 3 (MAPK3) ELISA Kit
RK01326

Human Mitogen-activated protein kinase 3 (MAPK3) ELISA Kit

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)