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[KD Validated] eIF4E Rabbit mAb (A25608)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Monkey

ABclonal:Western blot - [KD Validated] eIF4E Rabbit mAb (A25608)

Western blot analysis of lysates from wild type (WT) and eIF4E knockdown (KD) 293T cells using [KD Validated] eIF4E Rabbit mAb (A25608) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KD Validated] eIF4E Rabbit mAb (A25608)

Western blot analysis of various lysates using [KD Validated] eIF4E Rabbit mAb (A25608) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - [KD Validated] eIF4E Rabbit mAb (A25608)

Confocal imaging of 293T cells using [KD Validated] eIF4E Rabbit mAb (A25608, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] eIF4E Rabbit mAb (A25608)

Confocal imaging of Hep G2 cells using [KD Validated] eIF4E Rabbit mAb (A25608, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] eIF4E Rabbit mAb (A25608)

Confocal imaging of NIH/3T3 cells using [KD Validated] eIF4E Rabbit mAb (A25608, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] eIF4E Rabbit mAb (A25608)

Confocal imaging of PC-12 cells using [KD Validated] eIF4E Rabbit mAb (A25608, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunoprecipitation - [KD Validated] eIF4E Rabbit mAb (A25608)

Immunoprecipitation of [KD Validated] eIF4E in 200 µg extracts from 293T cells using 0.5 µg [KD Validated] eIF4E Rabbit mAb (A25608). Western blot analysis was performed using [KD Validated] eIF4E Rabbit mAb (A25608) at 1:3000 dilution.

Overview

Product name[KD Validated] eIF4E Rabbit mAb
Catalog No.A25608
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC66295
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4E (NP_001959.1).
SequenceMATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEP
Gene ID
Swiss Prot
SynonymsCBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
Calculated MW25kDa/27kDa/29kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat, Monkey
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:3000 - 1:18000
  • IF/ICC 1:200 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T, HeLa, COS-7
Cellular locationCytoplasm, P-body, Stress granule, Cytosol, Nucleus, P-body, chromatoid body, cytoplasm, cytoplasmic ribonucleoprotein granule, cytoplasmic stress granule, cytosol, eukaryotic translation initiation factor 4F complex, extracellular exosome, glutamatergic synapse, nucleus, perinuclear region of cytoplasm, postsynaptic cytosol.

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KD Validated] eIF4E Rabbit mAb (A25608)}

Western blot - [KD Validated] eIF4E Rabbit mAb (A25608)

Western blot analysis of lysates from wild type (WT) and eIF4E knockdown (KD) 293T cells using [KD Validated] eIF4E Rabbit mAb (A25608) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KD Validated] eIF4E Rabbit mAb (A25608)}

Western blot - [KD Validated] eIF4E Rabbit mAb (A25608)

Western blot analysis of various lysates using [KD Validated] eIF4E Rabbit mAb (A25608) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - [KD Validated] eIF4E Rabbit mAb (A25608)}

Immunofluorescence - [KD Validated] eIF4E Rabbit mAb (A25608)

Confocal imaging of 293T cells using [KD Validated] eIF4E Rabbit mAb (A25608, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] eIF4E Rabbit mAb (A25608)}

Immunofluorescence - [KD Validated] eIF4E Rabbit mAb (A25608)

Confocal imaging of Hep G2 cells using [KD Validated] eIF4E Rabbit mAb (A25608, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] eIF4E Rabbit mAb (A25608)}

Immunofluorescence - [KD Validated] eIF4E Rabbit mAb (A25608)

Confocal imaging of NIH/3T3 cells using [KD Validated] eIF4E Rabbit mAb (A25608, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] eIF4E Rabbit mAb (A25608)}

Immunofluorescence - [KD Validated] eIF4E Rabbit mAb (A25608)

Confocal imaging of PC-12 cells using [KD Validated] eIF4E Rabbit mAb (A25608, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunoprecipitation - [KD Validated] eIF4E Rabbit mAb (A25608)}

Immunoprecipitation - [KD Validated] eIF4E Rabbit mAb (A25608)

Immunoprecipitation of [KD Validated] eIF4E in 200 µg extracts from 293T cells using 0.5 µg [KD Validated] eIF4E Rabbit mAb (A25608). Western blot analysis was performed using [KD Validated] eIF4E Rabbit mAb (A25608) at 1:3000 dilution.

* For research use only. Not for therapeutic or diagnostic purposes.

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