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Phospho-eIF4E-S209 Rabbit mAb (AP1024)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - Phospho-eIF4E-S209 Rabbit mAb (AP1024)

Western blot analysis of lysates from HeLa cells, using Phospho-eIF4E-S209 Rabbit mAb (AP1024) at 1:1000 dilution. HeLa cells were treated by 20% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product namePhospho-eIF4E-S209 Rabbit mAb
Catalog No.AP1024
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1569
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
ImmunogenA phospho specific peptide corresponding to residues surrounding S209 of human Phospho-eIF4E-S209 (NP_001959.1).
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsCBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1; Phospho-eIF4E-S209
Calculated MW25kDa
Observed MW25kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa treated by FBS
Cellular locationCytoplasm, P-body, Cytoplasm, Cytoplasm, Stress granule, Cytosol, Nucleus.

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ABclonal:Western blot - Phospho-eIF4E-S209 Rabbit mAb (AP1024)}

Western blot - Phospho-eIF4E-S209 Rabbit mAb (AP1024)

Western blot analysis of lysates from HeLa cells, using Phospho-eIF4E-S209 Rabbit mAb (AP1024) at 1:1000 dilution. HeLa cells were treated by 20% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

* For research use only. Not for therapeutic or diagnostic purposes.

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