Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse
Western blot analysis of lysates from wild type (WT) and GLOD4 knockout (KO) HeLa cells, using [KO Validated] GLOD4 Rabbit pAb (A19849) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
| Product name | [KO Validated] GLOD4 Rabbit pAb |
|---|---|
| Catalog No. | A19849 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 179-298 of human GLOD4 (NP_057164.3). |
|---|---|
| Sequence | DNQCKLELQGVKGGVDHAAAFGRIAFSCPQKELPDLEDLMKRENQKILTPLVSLDTPGKATVQVVILADPDGHEICFVGDEAFRELSKMDPEGSKLLDDAMAADKSDEWFAKHNKPKASG |
| Gene ID | |
| Swiss Prot | |
| Synonyms | HC6; HC71; CGI-150; C17orf25; D4 |
| Calculated MW | 35kDa |
| Observed MW | 33kDa |
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HeLa |
| Cellular location | Mitochondrion |
* For research use only. Not for therapeutic or diagnostic purposes.
![ABclonal:Western blot - [KO Validated] GLOD4 Rabbit pAb (A19849)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A19849_H1761(KO)_KO-WB_01.jpg?t=1709190791 "ABclonal:Western blot - [KO Validated] GLOD4 Rabbit pAb (A19849)")
