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[KO Validated] N-Cadherin Rabbit pAb (A21308)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit pAb (A21308)

Western blot analysis of various lysates using [KO Validated] N-Cadherin Rabbit pAb (A21308) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit pAb (A21308)

Western blot analysis of lysates from HeLa cells, using [KO Validated] N-Cadherin Rabbit pAb (A21308) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit pAb (A21308)

Western blot analysis of lysates from 293T cells, using [KO Validated] N-Cadherin Rabbit pAb (A21308) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit pAb (A21308)

Western blot analysis of lysates from wild type(WT) and N-Cadherin knockout (KO) HeLa cells, using [KO Validated] N-Cadherin Rabbit pAb (A21308) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit pAb (A21308)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] N-Cadherin Rabbit pAb (A21308) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit pAb (A21308)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using [KO Validated] N-Cadherin Rabbit pAb (A21308) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunoprecipitation - [KO Validated] N-Cadherin Rabbit pAb (A21308)

Immunoprecipitation analysis of 600 μg extracts of Mouse brain using 3 μg N-Cadherin antibody (A21308). Western blot was performed from the immunoprecipitate using N-Cadherin antibody (A21308) at a dilution of 1:1000.

Overview

Product name[KO Validated] N-Cadherin Rabbit pAb
Catalog No.A21308
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 807-906 of human N-Cadherin (NP_001783.2).
SequenceRRMDERPIHAEPQYPVRSAAPHPGDIGDFINEGLKAADNDPTAPPYDSLLVFDYEGSGSTAGSLSSLNSSSSGGEQDYDYLNDWGPRFKKLADMYGGGDD
Gene ID
Swiss Prot
SynonymsCDHN; NCAD; ACOGS; ADHD8; CD325; ARVD14; CDw325; in
Calculated MW100kDa
Observed MW140kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesHeLa, 293T
Cellular locationCell membrane, Single-pass type I membrane protein.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit pAb (A21308)}

Western blot - [KO Validated] N-Cadherin Rabbit pAb (A21308)

Western blot analysis of various lysates using [KO Validated] N-Cadherin Rabbit pAb (A21308) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit pAb (A21308)}

Western blot - [KO Validated] N-Cadherin Rabbit pAb (A21308)

Western blot analysis of lysates from HeLa cells, using [KO Validated] N-Cadherin Rabbit pAb (A21308) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit pAb (A21308)}

Western blot - [KO Validated] N-Cadherin Rabbit pAb (A21308)

Western blot analysis of lysates from 293T cells, using [KO Validated] N-Cadherin Rabbit pAb (A21308) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit pAb (A21308)}

Western blot - [KO Validated] N-Cadherin Rabbit pAb (A21308)

Western blot analysis of lysates from wild type(WT) and N-Cadherin knockout (KO) HeLa cells, using [KO Validated] N-Cadherin Rabbit pAb (A21308) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit pAb (A21308)}

Immunohistochemistry - [KO Validated] N-Cadherin Rabbit pAb (A21308)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] N-Cadherin Rabbit pAb (A21308) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit pAb (A21308)}

Immunohistochemistry - [KO Validated] N-Cadherin Rabbit pAb (A21308)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using [KO Validated] N-Cadherin Rabbit pAb (A21308) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunoprecipitation - [KO Validated] N-Cadherin Rabbit pAb (A21308)}

Immunoprecipitation - [KO Validated] N-Cadherin Rabbit pAb (A21308)

Immunoprecipitation analysis of 600 μg extracts of Mouse brain using 3 μg N-Cadherin antibody (A21308). Western blot was performed from the immunoprecipitate using N-Cadherin antibody (A21308) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

재조합 단백질 (1)

Secondary Antibodies (24)