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[KO Validated] PRMT4/CARM1 Rabbit pAb (A2246)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246)

Western blot analysis of lysates from wild type (WT) and PRMT4/CARM1 knockout (KO) 293T cells, using [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246)

Immunohistochemistry analysis of paraffin-embedded Human colon using [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name[KO Validated] PRMT4/CARM1 Rabbit pAb
Catalog No.A2246
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 486-603 of human PRMT4/CARM1 (NP_954592.1).
SequenceGSHYTSPSENMWNTGSTYNLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGSSGAQGSGGGSTSAHYAVNSQFTMGGPAISMASPMSIPTNT
Gene ID
Swiss Prot
SynonymsPRMT4; M1
Calculated MW66kDa
Observed MW63kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T
Cellular locationCytoplasm, Nucleus.

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246)}

Western blot - [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246)

Western blot analysis of lysates from wild type (WT) and PRMT4/CARM1 knockout (KO) 293T cells, using [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246)}

Immunohistochemistry - [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246)

Immunohistochemistry analysis of paraffin-embedded Human colon using [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246)}

Immunohistochemistry - [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246)}

Immunohistochemistry - [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using [KO Validated] PRMT4/CARM1 Rabbit pAb (A2246) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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