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Phospho-PRMT4/CARM1-S228 Rabbit pAb (AP0319)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from Jurkat cells, using Phospho-PRMT4/CARM1-S228 Rabbit pAb (AP0319) at 1:1000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product name	Phospho-PRMT4/CARM1-S228 Rabbit pAb
Catalog No.	AP0319
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S228 of human PRMT4/CARM1 (NP_954592.1).
Sequence	VKSNN
Gene ID
Swiss Prot
Synonyms	PRMT4; Phospho-PRMT4/CARM1-S228
Calculated MW	66kDa
Observed MW	63kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Jurkat treated by Calyculin A
Cellular location	Cytoplasm, Nucleus.