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[KO Validated] SMARCC1/BAF155 Rabbit pAb (A6128)

KO/KDValidated

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - [KO Validated] SMARCC1/BAF155 Rabbit pAb (A6128)

Western blot analysis of lysates from wild type (WT) and SMARCC1/BAF155 knockout (KO) 293T cells, using [KO Validated] SMARCC1/BAF155 Rabbit pAb (A6128) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunoprecipitation - [KO Validated] SMARCC1/BAF155 Rabbit pAb (A6128)

Immunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg SMARCC1/BAF155 antibody (A6128). Western blot was performed from the immunoprecipitate using SMARCC1/BAF155 antibody (A6128) at a dilution of 1:1000.

Overview

Product name[KO Validated] SMARCC1/BAF155 Rabbit pAb
Catalog No.A6128
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 80-320 of human SMARCC1/BAF155 (NP_003065.3).
SequenceLVVQLLQFQEDAFGKHVTNPAFTKLPAKCFMDFKAGGALCHILGAAYKYKNEQGWRRFDLQNPSRMDRNVEMFMNIEKTLVQNNCLTRPNIYLIPDIDLKLANKLKDIIKRHQGTFTDEKSKASHHIYPYSSSQDDEEWLRPVMRKEKQVLVHWGFYPDSYDTWVHSNDVDAEIEDPPIPEKPWKVHVKWILDTDIFNEWMNEEDYEVDENRKPVSFRQRISTKNEEPVRSPERRDRKASA
Gene ID
Swiss Prot
SynonymsHYC5; Rsc8; SRG3; SWI3; BAF155; CRACC1; 55
Calculated MW123kDa
Observed MW155kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samples293T
Cellular locationNucleus
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] SMARCC1/BAF155 Rabbit pAb (A6128)}

Western blot - [KO Validated] SMARCC1/BAF155 Rabbit pAb (A6128)

Western blot analysis of lysates from wild type (WT) and SMARCC1/BAF155 knockout (KO) 293T cells, using [KO Validated] SMARCC1/BAF155 Rabbit pAb (A6128) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunoprecipitation - [KO Validated] SMARCC1/BAF155 Rabbit pAb (A6128)}

Immunoprecipitation - [KO Validated] SMARCC1/BAF155 Rabbit pAb (A6128)

Immunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg SMARCC1/BAF155 antibody (A6128). Western blot was performed from the immunoprecipitate using SMARCC1/BAF155 antibody (A6128) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A6128? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)