제품 > 항체 > 단일클론항체(mAb)

Ku80 Rabbit mAb (A12338)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates, using Ku80 Rabbit mAb (A12338) at 1：1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - Ku80 Rabbit mAb (A12338)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using Ku80 Rabbit mAb (A12338) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - Ku80 Rabbit mAb (A12338)

Confocal imaging of HeLa cells using Ku80 Rabbit mAb (A12338, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

ABclonal:Immunoprecipitation - Ku80 Rabbit mAb (A12338)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg Ku80 antibody (A12338). Western blot was performed from the immunoprecipitate using Ku80 antibody (A12338) at a dilution of 1:1000.

Overview

Product name	Ku80 Rabbit mAb
Catalog No.	A12338
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0706

Background

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 633-732 of human Ku80 (P13010).
Sequence	MKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQLNHFWEIVVQDGITLITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI
Gene ID
Swiss Prot
Synonyms	KU80; KUB2; Ku86; NFIV; KARP1; KARP-1; Ku80
Calculated MW	83kDa
Observed MW	86kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:6000 IHC-P 1:200 - 1:2000 IF/ICC 1:100 - 1:1000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	HeLa, A-549, MCF7
Cellular location	Chromosome, Nucleus, nucleolus.
Customer validation	WB(Homo sapiens)