LAMP1/CD107a Rabbit mAb (A23947)

Western blot analysis of lysates from C2C12 cells, using LAMP1/CD107a Rabbit mAb (A23947) at 1:5000 dilution. C2C12 cells were treated with PNGase F(1µL for 3h) at 37℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

Western blot analysis of lysates from NIH/3T3 cells, using LAMP1/CD107a Rabbit mAb (A23947) at 1:5000 dilution. NIH/3T3 cells were treated with PNGase F(1µL for 3h) at 37℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

Confocal imaging of NIH/3T3 cells using LAMP1/CD107a Rabbit mAb (A23947, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Flow cytometry: 1X10^6 RAW264.7 cells were surface-stained with LAMP1/CD107a Rabbit mAb (A23947, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained RAW264.7 cells were used as blank control (red line).

Flow cytometry: 1X10^6 RAW264.7 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or LAMP1/CD107a Rabbit mAb (A23947, 2 μg/mL, right).